不同产地山合欢皮HPLC指纹图谱的研究

建立山合欢皮正丁醇部位的指纹图谱。方法：采用HPLC法，色谱柱：Kromasil C18（250mm×4．6mm，5μm）；流动相：甲醇-乙腈-0．4％磷酸溶液，梯度洗脱；流速：1．0ml·min^-1；柱温：23℃；检测波长：210nm；参照物：icarside E5。结果：确定了25个共有峰，得到了以淫羊藿次苷E5为参照物的保留时间和相对峰面积，并利用《中药色谱指纹图谱相似度评价系统》计算相似度。结论：采用HPLC指纹图谱技术，可作为山合欢皮药材的质量控制。

Study on HPLC Fingerprints of Albizia Kalkora Prain from Different Area

To establish the HPLC fingerprints of Albizia kalkora prain from different area. Method： the HPLC separation was performed on a Kromasil C18 column （250mm × 4.6mm,5 μm）, gradient elution with mobile phase was methanol-acetonitrile -0.4% phosphoric acid solution at the flow rate of 1.0 ml·min^-1,the detection wavelength was 210 nm, the icariside E5 was reference standard. Result： Twenty-five co-possessing peaks were indicated, the retention time and relative peak area were obtained, the similar degrees was obtained from the similarity evaluation system for chromatographic fingerprint of TCM （version 2004 A）. Conclusion： The HPLC fingerprint method can be used in the quality control of Albizia kalkora prain.