不同类型胃息肉及胃癌中环氧化酶-2和P16蛋白的表达及相关性

目的:研究不同类型胃息肉及胃癌中COX-2(环氧化酶-2)和P16蛋白的表达,探讨COX-2、P16蛋白在胃癌发生、发展中的作用及其相互关系.方法:采用S-P免疫组织化学方法检测10例正常胃黏膜,30例非肿瘤性胃息肉(炎性及增生性胃息肉),20例肿瘤性胃息肉(腺瘤性胃息肉)和40例胃癌组织中,COX-2和P16蛋白的表达情况.结果:COX-2表达,在正常胃黏膜、非肿瘤性胃息肉、肿瘤性胃息肉及胃癌中分别为10%,13.33%,45%,75%,胃癌组阳性率与其他三组差异有显著性(P<0.05);P16蛋白表达,在正常胃黏膜、非肿瘤性胃息肉、肿瘤性胃息肉及胃癌中分别为90%,86.67%,60%,32.5%,胃癌组阳性率与其他三组差异有显著性(P<0.05).COX-2、P16蛋白表达与胃癌分化程度、淋巴结转移、呈一定相关性.COX-2与P16蛋白的表达呈负性相关(P<0.05).结论:COX-2、P16蛋白在胃癌发生、发展中起一定作用,可作为判定胃癌生物学行为的有用指标.

Expression of COX-2 and P16 protein and their correlations in various types of gastric polyps and gastric cancer cell

AIM: To explore the expression of COX-2 and P16 in various types of gastric polyps and gastric cancer cell, and to investigate the relationship between COX-2 and P16 protein in the process of gastric cancer. METHODS: The expression of COX-2 and P16 pro- teins were detected in normal gastric mucosa (n = 10), non-tumor gastric polyps (n = 30, in? ammatory polypsand hyperplastic polyps), tumor gastric polyps (n = 20, adenomatous polyps), and gastric cancer (n = 40) by S-P immunohistochemical staining. RESULTS: In the normal gastric mucosa, non-tumor gastric polyps, tumor gastric polyps, and gastric can- cer cell, the expression rate of the COX-2 was 10%, 13.33%, 45%, and 75%, respectively; and the rate of P16 protein expression in those tissues was 90%, 86.67%, 60%, and 32.5%, respectively. The positive rates of COX-2 and P16 protein were both significantly different between gastric cancer and other tissues (P <0.05). The expression of COX-2 and P16 were associated with the differentiation degree of gastric cancer and lymph node metastasis. COX-2 expression was negatively related to P16 expression in gastric cancer (P <0.05). CONCLUSION: COX-2 and P16 protein play key roles in the development of gastric cancer, and can be used as important indexes in the research of the biological behavior of gastric cancer.