Effects of fasting on serial measurements of hyperpolarized [1‐13C]pyruvate metabolism in tumors |
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| Authors: | Eva M. Serrao Tiago B. Rodrigues Ferdia A. Gallagher Mikko I. Kettunen Brett W. C. Kennedy Sarah L. Vowler Keith A. Burling Kevin M. Brindle |
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| Affiliation: | 1. Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK;2. Department of Biochemistry, University of Cambridge, Cambridge, UK;3. Department of Radiology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, UK;4. A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland;5. Core Biochemical Assay Laboratory, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK |
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| Abstract: | Imaging of the metabolism of hyperpolarized [1‐13C]pyruvate has shown considerable promise in preclinical studies in oncology, particularly for the assessment of early treatment response. The repeatability of measurements of 13C label exchange between pyruvate and lactate was determined in a murine lymphoma model in fasted and non‐fasted animals. The fasted state showed lower intra‐individual variability, although the [1‐13C]lactate/[1‐13C]pyruvate signal ratio was significantly greater in fasted than in non‐fasted mice, which may be explained by the higher tumor lactate concentrations in fasted animals. These results indicate that the fasted state may be preferable for the measurement of 13C label exchange between pyruvate and lactate, as it reduces the variability and therefore should make it easier to detect the effects of therapy. © 2016 The Authors. NMR in Biomedicine published by John Wiley & Sons Ltd. |
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| Keywords: | repeatability hyperpolarization fasting pyruvate lymphoma cancer |
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