School of Pharmacy, Yancheng Teachers’ University, Yancheng Jiangsu 224051 People’s Republic of China.; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009 People’s Republic of China
Abstract:
Development of high performance fluorescent chemosensors for the detection of ClO−in vitro and in vivo is very desirable, because many human diseases are caused by ClO−. In this paper, a highly selectivity and sensitive fluorescent probe, EDPC, based on 3-acetylcoumarin, was synthesized, which could respond to ClO− and exhibit an “off–on” mode in Tris–HCl buffer (pH = 7.2, 10 mM, 50% C2H5OH) solutions. The detection limit of the EDPC probe for ClO− was as low as 1.2 × 10−8 M. Moreover, the high selectivity and high sensitivity of EDPC towards ClO− are attributed to the oxidation reaction between the C–O of the coumarin lactone and the CC formed by aldol condensation and the mechanism was further verified using ESI-MS and DFT. Additionally, the concentrations of ClO− in real water were also calculated using the EDPC probe and showed good recovery. Finally, the distribution of intracellular endogenous ClO− was gained by confocal fluorescence microscopy in living HEK293T cells.A new probe, EDPC, was designed and synthesized for highly specific and sensitive detection of ClO− with a fast response time in living cells.
