A fluorescence polarization immunoassay for detection of thiacloprid in environmental and agricultural samples |
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| Authors: | Yuan Ding He Chen Qian Yang Lu Feng Xiude Hua Minghua Wang |
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| Affiliation: | College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 China, Fax: +86 25 84395479, +86 25 84395479 ; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095 China |
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| Abstract: | As a widely used neonicotinoid insecticide, thiacloprid has been observed to pose a risk to honeybees and the endocrine system of mammals. So a detection method with high sensitivity, simple operation and high throughput is required. Based on this consideration, we prepared an anti-thiacloprid monoclonal antibody (mAb, C9) and developed a fluorescence polarization immunoassay (FPIA) for the detection of thiacloprid. After optimizing the length of spacer and reaction conditions, the 50% inhibition concentration (IC50), limit of detection (LOD) and linear range (IC20 ∼ IC80) of the FPIA are 15.34 ng mL−1, 2.43 ng mL−1 and 3.10–65.7 ng mL−1, respectively. Meanwhile, FPIA just requires 12 min to detect the pesticide with simple operation. Then the FPIA was used to detect the thiacloprid in spiked rice, soil, cucumber and tomato samples, and recoveries were in the range of 79.1%–105.3% with 3.7%–12.3% standard deviation. The FPIA also shows good correlation with high-performance liquid chromatography for the detection of thiacloprid in tomato samples.An anti-thiacloprid monoclonal antibody with high sensitivity was prepared and used to develop a fluorescence polarized immunoassay. |
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