Mitochondrial DNA typing from human axillary, pubic and head hair shafts – success rates and sequence comparisons |
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Authors: | H Pfeiffer J Hühne C Ortmann K Waterkamp B Brinkmann |
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Institution: | (1) Institute of Forensic Medicine, University of Münster, von Esmarch Strasse 62, D-48149 Münster, Germany, DE |
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Abstract: | The analysis of mitochondrial DNA (mtDNA) from shed hairs has gained high importance in forensic casework since telogen hairs
are one of the most common types of evidence left at the crime scene. In this systematic study of hair shafts from 20 individuals,
the correlation of mtDNA recovery with hair morphology (length, diameter, volume, colour), with sex, and with body localisation
(head, armpit, pubis) was investigated. The highest average success rate of hypervariable region 1 (HV 1) sequencing was found
in head hair shafts (75%) followed by pubic (66%) and axillary hair shafts (52%). No statistically significant correlation
between morphological parameters or sex and the success rate of sequencing was found. MtDNA sequences of buccal cells, head,
pubic and axillary hair shafts did not show intraindividual differences. Heteroplasmic base positions were observed neither
in the hair shafts nor in control samples of buccal cells.
Received: 18 January 1999 / Accepted: 15 February 1999 |
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Keywords: | Mitochondrial DNA Hypervariable region 1 Head axillary and pubic hair Shafts Heteroplasmy |
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