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| 马钱子生物碱血浆蛋白结合率的测定与比较 | |
| 引用本文： | 王绚,何超芹,陈亚,陈军,蔡宝昌.马钱子生物碱血浆蛋白结合率的测定与比较[J].中国中药杂志,2011,36(2):185-188. |
| 作者姓名： | 王绚 何超芹 陈亚 陈军 蔡宝昌 |
| 作者单位： | 1. 南京中医药大学,药学院,江苏,南京,210046;江苏省中药炮制重点实验室,江苏,南京,210029 2. 南京中医药大学,药学院,江苏,南京,210046 3. 江苏省中药炮制重点实验室,江苏,南京,210029 |
| 基金项目： | 国家自然科学基金项目(30701111);江苏省大学生创新训练计划项目(380) |
| 摘 要： | 目的:测定马钱子总生物碱中主要有效成分马钱子碱和士的宁的血浆蛋白结合率,并与同浓度单成分的血浆蛋白结合率进行比较.方法:采用超滤法和高效液相色谱法对马钱子生物碱在大鼠血浆中的血浆蛋白结合率进行测定.结果.单成分的马钱子碱在0.520,1.300,2.600 mg·L-1下的血浆蛋白结合率分别为(65.60±3.01)%,(68.20±7.80)%,(59.58±3.78)%.单成分的士的宁在0.936,2.340,4.680 nag·L-1下的血浆蛋白结合率分别为(66.17±6.36)%,(67.10±2.52)%,(57.21±0.79)%.马钱子总生物碱中马钱子碱在0.519,1.288,2.607 nag·L-1下的血浆蛋白结合率分别为(62.19±2.45)%,(69.55±5.84)%,(61.76±3.68)%;马钱子总生物碱中士的宁在0.940,2.338,4.674 nag·L-1下的血浆蛋白结合率分别为(54.79±3.55)%,(57.13±4.49)%,(59.31±3.65)%.结论:马钱子碱和士的宁与血浆蛋白具有中等强度的结合,马钱子总生物碱中马钱子碱与同浓度单体相比,蛋白结合率差异不大;总碱中士的宁与同浓度单体相比,蛋白结合率有所降低. |
| 关 键 词： | 马钱子碱 士的宁 马钱子总生物碱 血浆蛋白结合率 超滤法 高效液相色谱法 |
| 收稿时间： | 2010/6/18 0:00:00 |
Determination and comparison of plasma protein binding rate of alkaloids from seed of Strychnou nux-vomica |
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| WANG Xuan,HE Chaoqin,CHEN Y,CHEN Jun and CAI Baochang.Determination and comparison of plasma protein binding rate of alkaloids from seed of Strychnou nux-vomica[J].China Journal of Chinese Materia Medica,2011,36(2):185-188. | |
| Authors: | WANG Xuan HE Chaoqin CHEN Y CHEN Jun and CAI Baochang |
| Institution: | College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210029, China;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210029, China;Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210029, China |
| Abstract: | Objective : To determine the plasma protein binding rates of brucine and strychnine in total alkaloids from the seed of Strychnou nux-vomica, and make comparison with the single components at the same concentration. Method : Ultrafiltration was employed to determine the rat the plasma protein binding rate of the alkaloids from the seed of S. nux-vomica. The plasma concentrations were measured by RP-HPLC. Result : The protein binding rates of brucine were (65.60 3.01)%,(68.20±7.80)%, (59.58±3.78)% when the plasma concentrations was 0.520,1.300,2.600 mg·L-1, respectively. The protein binding rates of strychnine was (66.17±6.36)%,(67.10±2.52)%,(57.21±0.79)% when the plasma concentrations were 0.936,2.340,4.680 mg·L-1 respectively. As to the total alkaloids from the seed of S. nux-vomica, The protein binding rate of brucine was (62.19±2.45)%,(69.55±5.84)%,(61.76±3.68)% when the plasma concentrations were 0.519,1.288,2.607 mg·L-1, respectively. And the protein binding rates of strychnine were (54.79±3.55)%,(57.13±4.49)%,(59.31±3.65)% when the plasma concentrations were 0.940,2.338,4.674 mg·L-1, respectively. Conclusion : Brucine and strychnine have medium capacity in binding to plasma protein. In comparison with the single component of the same concentration, the protein binding rate of brucine in total alkaloids shows little difference, while there seems to be an obvious decrease for strychnine. |
| Keywords: | brucine strychnine total alkaloids plasma protein binding rate ultrafiltration HPLC |
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