盐酸克仑特罗对小鼠胚胎体外发育的影响

目的:探讨盐酸克仑特罗对小鼠1-细胞胚胎和2-细胞胚胎体外发育的影响。方法:获取小鼠1-细胞和2-细胞胚胎,分别与盐酸克仑特罗10 ng/mL,3 ng/mL和1 ng/mL的3个剂量组共培养,观察胚胎各阶段的发育情况并计算胚胎的发育率。结果:1ng/mL和3ng/mL组的1-细胞小鼠胚胎,从4-细胞期到囊胚阶段的发育率与对照组相比差异显著(P<0.05),3 ng/mL组显现出比1ng/mL组更强的抑制作用(P<0.01),10 ng/mL组,2-细胞期就与对照组有差异(P<0.05,其中 4-细胞至囊胚阶段P<0.01),囊胚率只有2.4％。10 g/mL组及3 ng/mL组的2-细胞小鼠胚胎,分别从4-细胞期和8-细胞期与对照组相比差异显著(P<0.05),1 ng/mL组的各个阶段胚胎发育率与对照组相比未见统计学差异(P>0.05)。培养液中含有盐酸克仑特罗使胚胎的粗颗粒增多,部分印裂球碎裂、退化。结论:盐酸克仑特罗对小鼠胚胎的体外发育有毒性作用并呈一定的剂量效应。盐酸克仑特罗使1-细胞小鼠胚胎被抑制在2-细胞期,对处于晚2-细胞期胚胎的影响明显降低。

Effect of Clenbuterol Hydrochloride on the in vitro Development of 1-cell and 2-cell Mouse Embryos

Objective:To investigate the effect of clenbuterol hydrochloride on the in vitro development of both 1-cell and 2-cell mouse embryos. Methods :The in vitro cultural systems of both 1-cell and 2-cell mouse embryo were used to determine the effect of clenbuterol hydrochloride at doses of 1 ng/mL, 3 ng/mL and 10 ng/mL on developmental rates of mouse embryos. Results: (1) When 1-cell embryos cultured with 1 ng/ mL of clenbuterol hydrochloride, developmental rates from 4-cell to blastocyst stage were significantly lower than those in the control group (P<0. 05). With doses of 3 ng/mL and 10 ng/mL, the inhibiting effects on embryo development were significantly increased (F<0. 01). (2) When 2-cell embryos cultured with 1 ng/mL of clenbuterol hydrochloride, obvious differences in developmental rates were not found between the 2-cell embryo group and the control (P>0. 05). However, at levels of 3 ng/mL and 10 ng/mL, significant decrease of developmental rates in 2-cell embryos was observed from 4-cell and from 8-cell stage, respectively (P<0. 05). (3) Embryos cultured with clenbuterol hydrochloride had more granules, fragments and degeneration than those in the control. Conclusion: Clenbuterol hydrochloride has a toxic effect on the mouse embryos, and the effect is in a dose-dependent. 1-cell mouse embryos cultured with clenbuterol hydrochloride could be easily inhibited at 2-cell stage, but the effect of clenbuterol hydrochloride on development of the late 2-cell embryos would be reduced.