二氢埃托啡自动静脉给药对大鼠免疫功能的影响

本研究采用计算机实时控制的定时自动静脉给药法，７２ｈ快速致使大鼠身体依赖性形成。各组戒断症状分值为：ＮＳ组１１．０±ｓ２．９；吗啡组８１．０±ｓ８．６；ＤＨＥ组４１．０±ｓ４．４，表明大鼠已形成明显身体依赖性（Ｐ＜０．００１）。在该模型基础上，利用３Ｈ－ＴｄＲ掺入法测得ＤＨＥ明显抑制大鼠淋巴细胞增殖反应。各组刺激指数为：８０．６９±ｓ１．７６；４９．０４±ｓ１．１０和３０．７７±ｓ２．１４（Ｐ＜０．００１）。利用ＭＴＴ法测ＤＨＥ也能抑制大鼠ＩＬ－２活性，各组Ａ５７０ｎｍ值为：０．３９±ｓ０．０３；０．３１±ｓ０．０４和０．２６±ｓ０．０４（Ｐ＜０．０１）。结果表明ＤＨＥ自动静脉给药明显抑制大鼠免疫功能，且较１０００倍剂量的吗啡作用更强

EFFECT OF DIHYDROETORPHINE ON IMMUNE FUNCTIONS IN RATS TREATED BY AUTOMATICAL INTRAVENOUS INJECTION

Computer controlled automatical intravenous injection was adopted to establish physical dependence in DHE treated rats. The withdrawal scores of 2 opiate-dependent rat groups were: DHE group 41.0±s 4.4; Morphine group 81.0±s 8.6. Both showed significant differences compared with that of NS group (11.0±s 2.9, P< 0.001).Based on this modal, effects of DHE on immune functions were examined. DHE significantly suppresses the Con-A activated splenic lymphocyte proliferations in rats. The stimulation indexes were: DHE group 30.77±s 2.14; Morphine group 49.04±s 1.10.Both showed significant differences compared with that of NS group (80.67±s 1.76, P< 0.001).DHE can also suppress the activity of IL-2 in the supernatant of Con-A activated splenocytes in rats. The values of A570nm were: DHE group 0.26±s 0.04; Morphine group 0.31±s 0.04. Both showed differences compared with that of NS group (0.39±s 0.03, P<0.01).The results indicate that DHE has powerful immunosuppressive effects in rats; furthermore, its effects are at least as 1000 times as those of morphine at the same dose.