牛膝多糖衍生物对糖尿病大鼠胰岛细胞形态和肾功能的影响

目的：探讨牛膝多糖（AbP）及其硫酸酯和磷酸酯衍生物对糖尿病（DM）大鼠胰岛细胞形态和肾功能的影响。方法：以链脲佐菌素诱导SD大鼠致DM模型,观察AbP、牛膝多糖硫酸酯衍生物（S-AbP）和牛膝多糖磷酸酯衍生物（P-AbP）对DM大鼠血糖、胰岛细胞形态和分泌的影响;测定血清肌酐（Scr）和尿素氮（BUN）水平,观察其对DM大鼠肾功能的影响。结果：AbP及其两种衍生物均可明显降低DM大鼠的血糖浓度（P〈0.05或P〈0.01）,S-AbP和P-AbP可明显减轻DM大鼠胰岛β细胞损伤,使胰岛素阳性反应细胞增多;S-AbP显著降低Scr和BUN水平（均P〈0.01）,P-AbP和AbP显著降低Scr水平（均P〈0.01）。结论：S-AbP和P-AbP对高血糖所致胰岛β细胞损伤有明显的保护和修复作用,对肾功能有不同程度的改善作用。

Effects of the derivatives of Achyranthes bidentata Polysaccharides on the morphology of islet cellular morphology and renal function in diabetic rats

Objective： To study the effects of Achyranthes bidentata polysaccharides （AbP） and the sulfated derivative （S-AbP） and the phosphorylated derivative （P-AbP） of AbP on the morphology of islet cells and renal function in diabetic rats.Methods： Diabetes was induced by a single injection of streptozotocin,changes of fasting blood glucose and morphology and secretion of islet cells in diabetic rats treated respectively with S-AbP,P-AbP and AbP were observed,the renal function was also evaluated by examining serum creatinine （Scr） and blood urea nitrogen （BUN）.Results： Compared with diabetes group,the blood glucose significantly decreased in S-AbP,P-AbP and AbP treated group （P 0.05 or P 0.01）,and the effect of S-AbP on reducing blood glucose was better than that of AbP （P 0.01）;S-AbP and P-AbP significantly lessen pathological damage,and increase islet cells positive to insulin ;S-AbP significantly degraded BUN and Scr level （P 0.01 respectively） ;P-AbP and AbP significantly degraded Scr level （P 0.01 respectively）.Conclusion： S-AbP and P-AbP exert a good protective and reparative effect on damages of islet cells and can improve renal function of diabetic rats in some degree.