| 夏枯草愈伤组织诱导和植株再生技术的研究 |
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| 引用本文： | 盛玮,常莉,张爱民,薛建平,刘玉玲.夏枯草愈伤组织诱导和植株再生技术的研究[J].中国中药杂志,2010,35(7):830-833. |
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| 作者姓名： | 盛玮 常莉 张爱民 薛建平 刘玉玲 |
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| 作者单位： | 淮北煤炭师范学院,生命科学学院,资源植物生物学安徽省重点实验室,安徽,淮北,235000 |
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| 基金项目： | 安徽省教育厅省级自然科学研究项目(KJ2009B2532);资源植物生物学安徽省重点实验室开放基金项目(KLPRB200913) |
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| 摘 要： | 目的:探讨夏枯草愈伤组织诱导和植株再生条件。方法:采用正交试验法研究不同外植体、蔗糖、植物生长物质及其配比对夏枯草愈伤组织诱导和分化的影响。结果和结论:以叶片为外植体诱导愈伤效果最好,茎段次之,叶柄不能诱导出愈伤组织;6-BA是影响夏枯草愈伤组织形成的主要因素;诱导愈伤组织的适宜培养基为MS+6-BA 3.0 mg.L-1+NAA 0.1mg.L-1+2,4-D 0.5 mg.L-1+蔗糖3%;宜于愈伤组织分化的培养基为1/2 MS+6-BA 3.25 mg.L-1+NAA1.25 mg.L-1+蔗糖2%;最佳生根培养基:MS+IBA 1.0 mg.L-1+蔗糖3%。
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| 关 键 词： | 夏枯草 组织培养 愈伤组织 分化 植株再生 |
| 收稿时间： | 2009/9/28 0:00:00 |
Research on callus induction and plantlet regeneration of Prunella vulgaris |
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| SHENG Wei,CHANG Li,ZHANG Aimin,XUE Jianping and LIU Yuling.Research on callus induction and plantlet regeneration of Prunella vulgaris[J].China Journal of Chinese Materia Medica,2010,35(7):830-833. |
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| Authors: | SHENG Wei CHANG Li ZHANG Aimin XUE Jianping and LIU Yuling |
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| Institution: | Anhui Key Laboratory of Plant Resources and Biology, School of Life Science,Huaibei Coal Industry Teachers' College, Huaibei 235000, China;Anhui Key Laboratory of Plant Resources and Biology, School of Life Science,Huaibei Coal Industry Teachers' College, Huaibei 235000, China;Anhui Key Laboratory of Plant Resources and Biology, School of Life Science,Huaibei Coal Industry Teachers' College, Huaibei 235000, China;Anhui Key Laboratory of Plant Resources and Biology, School of Life Science,Huaibei Coal Industry Teachers' College, Huaibei 235000, China;Anhui Key Laboratory of Plant Resources and Biology, School of Life Science,Huaibei Coal Industry Teachers' College, Huaibei 235000, China |
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| Abstract: | Objective : To study the conditions of callus induction and plantlet regeneration of Prunella vulgaris. Method : By using the orthogonal experiment design, different explants, sucrose, plant growth substances and their ratio for callus induction and differentiation were optimized. Result and Conclusion : The inductivity of leaves was the highest, followed by stems,but callus of the leafstalks could not be induced. The main factor of affecting the formation of callus was 6-BA. The optimal medium for callus induction was MS+6-BA 3.0 mg·L-1 + NAA 0.1 mg·L-1 + 2,4-D 0.5 mg·L-1+ sucrose 3%. The optimal medium for callus differentiation was 1/2 MS+6-BA 3.25 mg·L-1 + NAA 1.25 mg·L-1 + sucrose 2%. The optimal medium for rooting was MS+IBA 1.0 mg·L-1+sucrose 3%. |
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| Keywords: | Prunella vulgaris tissue culture callus differentiation plantlet regeneration |
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