Antibacterial activity and mechanism of berberine against Streptococcus agalactiae
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| Authors: | Lianci Peng Shuai Kang Zhongqiong Yin Renyong Jia Xu Song Li Li Zhengwen Li Yuanfeng Zou Xiaoxia Liang Lixia Li Changliang He Gang Ye Lizi Yin Fei Shi Cheng Lv Bo Jing |
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| Affiliation: | 1College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China;2Institute of Prevention Veterinary Medicine, Sichuan Agricultural University, Chengdu, China |
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| Abstract: | The antibacterial activity and mechanism of berberine against Streptococcus agalactiae were investigated in this study by analyzing the growth, morphology and protein of the S. agalactiae cells treated with berberine. The antibacterial susceptibility test result indicated minimum inhibition concentration (MIC) of berberine against Streptococcus agalactiae was 78 μg/mL and the time-kill curves showed the correlation of concentration-time. After the bacteria was exposed to 78 μg/mL berberine, the fragmentary cell membrane and cells unequal division were observed by the transmission electron microscopy (TEM), indicating the bacterial cells were severely damaged. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) study demonstrated that berberine could damage bacterial cells through destroying cellular proteins. Meanwhile, Fluorescence microscope revealed that berberine could affect the synthesis of DNA. In conclusion, these results strongly suggested that berberine may damage the structure of bacterial cell membrane and inhibit synthesis of protein and DNA, which cause Streptococcus agalactiae bacteria to die eventually. |
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| Keywords: | Berberine Streptococcus agalactiae antibacterial activity SDS-PAGE TEM |
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