MiR-26b inhibits hepatocellular carcinoma cell proliferation,migration, and invasion by targeting EphA2 |
| |
| Authors: | Hesheng Li Qinglei Sun Bing Han Xingquan Yu Baoguang Hu Sanyuan Hu |
| |
| Institution: | 1Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China;2Department of Gastrointestinal Surgery, The Affiliated Hospital of Binzhou Medical University, Binzhou 256603, China;3College of Medicine and Nursing, Dezhou University, Dezhou 253023, China |
| |
| Abstract: | Deregulated microRNAs (miRNAs) have been shown to play important roles in cancer progression as a result of changes in expression of their target genes. In this study, we investigated the expression of miR-16b in eight hepatocellular carcinoma (HCC) cell lines, revealed the roles of miR-26b on hepatocellular carcinoma (HCC) cell proliferation, migration, and invasion, and confirmed that EphA2 is a direct target of miR-26b. The miR-26b expression was decreased and EphA2 expression was evaluated in HCC cell lines. Luciferase assays revealed that miR-26b inhibited EphA2 expression by targeting the 3’-untranslated region of EphA2 mRNA. Overexpression of miR-26b dramatically inhibited the proliferation, invasion, and migration of HCC cells by targeting EphA2. Moreover, miR-26b down-regulated c-Myc and CyclinD1 expression, which was reversed by overexpressed EphA2. Taken together, our data demonstrated the mechanism of miR-26b contributed to HCC progression and implicated that miR-26b’s potential in HCC therapy. |
| |
| Keywords: | miR-26b hepatocellular carcinoma EphA2 proliferation invasion migration |
|
|
