| 拉米夫定耐药后序贯治疗中HBV准种的演变 |
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| 引用本文： | 吴珍萍,韩涛,高英堂,李莹,刘彤,景丽,刘磊,杜智. 拉米夫定耐药后序贯治疗中HBV准种的演变[J]. 世界华人消化杂志, 2010, 18(8) |
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| 作者姓名： | 吴珍萍 韩涛 高英堂 李莹 刘彤 景丽 刘磊 杜智 |
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| 作者单位： | 天津医科大学三中心临床学院,天津市,300170;天津市第三中心医院天津市人工细胞重点实验室,天津市,300170 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)基金资助项目;No.2007CB512801科技部科技重大专项基金资助项目;No.2008ZXl0002-005天津市科委重点攻关专项基金资助项目;No.05YFSZSF02500天津市科委应用基础研究计划面上基金资助项目 |
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| 摘 要： | 目的:探讨拉米夫定(LAM)耐药后序贯治疗中HBV准种的演变特点.方法:收集7例拉米夫定耐药患者序贯治疗中的血清,对HBV聚合酶基因逆转录酶区进行PCR扩增克隆并测序,结合临床用药分析序列突变,探讨其演变特征.结果:患者1、4、5在序贯治疗中LAM耐药变异株一直存在,主要以M204I+L80I、M204I+L80I+L180M、M204V+L180M+G173L、M204V+L180M为主,所占比例在不断发生变化,患者2、3在序贯治疗中检测出野生株,但患者2在后续治疗中又选择出LAM耐药变异株.患者6在ADv联合ETV治疗时、患者7在单用ADV治疗时测时分别测出双重耐药株M204v+L180M+G173L+T184A和M204V+180M+G173L+236D.结论:拉米夫定耐药后序贯治疗中原耐药突变不易消失,且容易在此基础上筛选出交叉耐药株或多重耐药株,他的出现影响序贯治疗的应答甚至导致序贯治疗的失败.
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| 关 键 词： | 拉米夫定 耐药 乙型肝炎病毒 准种 |
Evolution of hepatitis B virus quasispecies during sequential therapy in patients with lamivudine-resistant hepatitis B virus infection |
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| Zhen-Ping Wu,Tao Han,Ying-Tang Gao,Ying Li,Tong Liu,Li ling,Lei Liu,Zhi Du. Evolution of hepatitis B virus quasispecies during sequential therapy in patients with lamivudine-resistant hepatitis B virus infection[J]. World Chinese Journal of Digestology, 2010, 18(8) |
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| Authors: | Zhen-Ping Wu Tao Han Ying-Tang Gao Ying Li Tong Liu Li ling Lei Liu Zhi Du |
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| Affiliation: | Zhen-Ping Wu,the Third Central Hospital,Tianjin Medical University,Tianjin 300170,China.Tao Han,Ying-Tang Gao,Ying Li,Tong Liu,Li Jing,Lei Liu,Zhi Du,Tianjin Key Laboratory of Artificial Cells,Tianjin Third Central Hospital,China |
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| Abstract: | AIM:To investigate the evolution characteristics of hepatitis B virus (HBV) quasispecies during sequential therapy in patients with lamivudineresistant HBV infection.METHODS:Serum samples were collected from seven patients with lamivudine-resistant HBV infection during sequential therapy.The reversetranscriptase (RT) region of the HBV polymerase gene was amplified,cloned and sequenced.Sequence mutations were analyzed to characterize the evolution of HBV quasispecies.RESULTS:Lamivudine-resistant strains were... |
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| Keywords: | Lamivudine Resistance Hepatitis B virus Quasispecies |
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