乙肝病毒感染者外周血CD4^＋CD25^＋调节性T细胞的表达及临床意义

目的 探讨乙肝病毒感染者外周血CD4 CD25 调节性T细胞(CD4 CD25 Tr)表达水平及其临床意义.方法 选择乙肝病毒携带者25例、急性乙肝患者26例、慢性乙肝患者38例,26例正常对照,用流式细胞仪检测外周血中CD4 CD25 Tr表达水平;用PCR法检测患者HBV DNA载量.结果 乙肝病毒携带者组与慢性乙肝患者组CD4 CD25 Tr水平(9.93±3.51,11.21±3.29)明显高于对照组(8.12±2.80)(P＜0.01或0.05),急性肝炎组与对照组相比,差异没有显著性.结论 持续性HBV感染者CD4 CD25 Tr表达增加,提示CD4 CD25 Tr在慢性乙型肝炎中担负着重要的免疫调节作用,可能抑制HBV感染者特异性细胞免疫反应,与乙肝病毒感染的慢性化及肝病的临床发生、发展有关.

Expression of CD4+ CD25+ Tr in the peripheral blood of hepatitis B virus in fection and its clinical significance

Objective To explore the expression of CD4 CD25 Tr in the peripheral blood of hepatitis B virus infection and its clinical significance.Methods Selection of asymptomatic carriers(ASC,n=25)acute hepatitis B(AHB,n=26),chronic hepatitis B(CHB,n=38) and normal subjects(n=26),CD4 CD25 Tr in peripheral blood of the four groups were detected by flow cytometry,the level of HBVDNA in serum were dynamically detected by PCR.Results CD4 CD25 Tr expression in ASC and CHB elevated significantly than that in normal subjects(P<0.05 or P<0.01).However,there is little discrepancy between AHB and normal subjects.Conclusion CD4 CD25 Tr in ASC and CHB is increased.The finding suggested that CD4 CD25 Tr play an active role in heptitis B virus infection,and relate clinical development.