海洋放线菌Kocuria sp.次级代谢产物的研究

目的 研究海洋放线菌Kocuria sp.的次级代谢产物。方法 菌株摇瓶发酵，采用现代色谱学方法(硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备HPLC)，对发酵产物进行分离，利用现代波谱学技术对化合物进行结构鉴定。结果 从海洋放线菌Kocuria sp.发酵液的乙酸乙酯萃取部分分离得到16个单体化合物：环(L-苯丙氨酸-L-脯氨酸)2 (1)、环(L-色氨酸-L-脯氨酸) (2)、环(L-色氨酸-D-脯氨酸) (3)、环(L-苯丙氨酸-D-脯氨酸) (4)、环(L-羟脯氨酸-L-苯丙氨酸) (5)、环(D-羟脯氨酸-L-苯丙氨酸) (6)、环(L-羟脯氨酸-L-酪氨酸) (7)、环(L-异亮氨酸-D-脯氨酸) (8)、环(L-亮氨酸-D-脯氨酸) (9)、环(L-亮氨酸-L-脯氨酸) (10)、环(L-苯丙氨酸-L-酪氨酸) (11)、环(L-亮氨酸-D-酪氨酸) (12)、环(L-亮氨酸-L-苯丙氨酸) (13)、环(D-缬氨酸-L-苯丙氨酸) (14)、环(D-亮氨酸-甘氨酸) (15)、环(D-异亮氨酸-甘氨酸) (16)。结论 海洋放线菌Kocuria sp.可产生结构丰富多样的环肽类化合物，所有化合物均首次从Kocuria属放线菌中分离得到。

Studies on the secondary metabolites of marine actinomycete Kocuria sp.

Objective To study the secondary metabolites of marine actinomycete Kocuria sp. Methods The strain was cultured in flasks. The fermentation products were separated by column chromatography (silica gel and Sephadex LH-20), and semi-preparative HPLC. The structures of the purified compounds were elucidated by spectroscopic analyses. Results Sixteen compounds were obtained from the ethyl acetate extracts and were identified as: cyclo(L-Phe-L-Pro)2 (1), cyclo(L-Trp-L-Pro) (2), cyclo(L-Trp-D-Pro) (3), cyclo(L-Phe-D-Pro) (4), cyclo(L-4-hydroxyl-Pro-L-Phe) (5), cyclo(D-4-hydroxyl-Pro-L-Phe) (6), cyclo(L-4-hydroxyl-Pro-L-Tyr) (7), cyclo(L-Ile-D-Pro) (8), cyclo(L-Leu-D-Pro) (9), cyclo(L-Leu-L-Pro) (10), cyclo(L-Phe-L-Tyr) (11), cyclo(L-Leu-D-Tyr) (12), cyclo(L-Leu-L-Phe) (13), cyclo(D-Val-L-Phe) (14), cyclo(D-Leu-Gly) (15), and cyclo(D-Ile-Gly) (16). Conclusion The marine actinomycete Kocuria sp. could produce cyclopeptides with diverse structures, and all the compounds were isolated from the genus Kocuria for the first time.