南极真菌Penicillium sp. S-2-10次级代谢产物的研究

目的 对来源于南极海域海底沉积物（47.09° W，62.05° S，水深1393 m）真菌Penicillium sp. S-2-10的次级代谢产物进行研究。方法 对菌株进行规模发酵，并对所得发酵液的乙酸乙酯萃取浸膏进行系统分离纯化，据化学指征和生物学活性两方面特点，采用Sephadex LH 20凝胶色谱、正相硅胶柱色谱、反相ODS柱色谱、高效液相柱层析等色谱分析方法，通过核磁（1H-NMR和13C-NMR等）及质谱（MS）等波谱分析方法，并与相关文献比较，鉴定化合物结构。 结果 分离得到7个单体化合物，经鉴定化合物1~7分别为Benzoic acid, 2-(2,6-dihydroxybenzoyl)-3-hydroxy-5-(hydroxymethyl)-methyl, methyl ester (1), chrysophanol (2), Volemolide (3), Ergosta-6,8(14),22-trien-3-ol (4), 2-aminophenoxazin-3-one (5), 2-benzothiazolol (6), benzoic acid (7)。结论 化合物3，4为首次从Penicillium属真菌中获得。

Research of the Secondary Metabolites from Antarctic-derived Fungus Penicillium sp. S-2-10

Objective To study the secondary metabolites of the fungus Penicillium sp. S-2-10, derived from the Antarctic seabed sediments (47.09° W, 62.05° S, the depth of 1393 m). Method The ethyl acetate extract of the fermented liquid extract was purified systematically combining chemical characteristics and bioassaies after large scale fermentation, using Sephadex LH 20 gel chromatography, normal phase silica gel column chromatography, reversed phase ODS column chromatography and high performance liquid chromatographic analysis methods. The structures of the compounds were identified on the basis of nuclear magnetic resonance (1H and 13C NMR), mass spectrometry (MS) spectrum and the related literature. Results Compounds 1~7 were identified as Benzoic acid, 2-(2,6-dihydroxybenzoyl)-3-hydroxy-5-(hydroxymethyl)-methyl, methyl ester (1), chrysophanol (2), Volemolide (3), Ergosta-6,8(14),22-trien-3-ol (4), 2-aminophenoxazin-3-one (5), 2-benzothiazolol (6), benzoic acid (7). Conclusion Compounds 3 and 4 were firstly isolated from the fungus Penicillium sp.