层析法去除基因重组 125Ser-IL-2产品中细菌内毒素 |
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引用本文: | 李莉,陶征宇,刘全海.层析法去除基因重组 125Ser-IL-2产品中细菌内毒素[J].中国医药工业杂志,2002,33(2):68-69. |
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作者姓名: | 李莉 陶征宇 刘全海 |
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作者单位: | 上海医药工业研究院,上海,200040 |
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摘 要: | 针对细菌内青素的化学性质,比较了用亲和层析法,离子交换层析法和疏水相互作用层析法等3种层析法去除基因重组^125Ser-IL-2产品中细菌内毒素的效果。结果表明,亲和层析法的效果最好,但综合各方面的因素,离子交换层析法更实用。
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关 键 词: | 多粘菌素亲和层析法 离子交换层析法 疏水相互作用层析法 细菌内毒素 ^125Ser-IL-2 |
文章编号: | 1001-8255(2002)02-0068-02 |
修稿时间: | 2001年9月28日 |
Removal of Bacterial Endotoxin by Chromatography in Purification of Recombinant 125Ser-IL-2 |
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Authors: | LI Li TAO Zheng- Yu LIU Quan- Hai |
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Abstract: | Based on the property of bacterial endotoxin,the effects of affinity chromatography,ion exchange chromatography and hydrophobic interaction chromatography on removal of endotoxin in the pu- rification of recombinant12 5Ser- IL - 2 were compared. Though PME affinity chromatography performed a best result,ion exchange chromatography was more reasonable because itcould also improve the purity of 12 5Ser- IL- 2 . |
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Keywords: | PME affinity chromatography ion exchange chromatography hydrophobic interaction chromatography bacterial endotoxin |
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