乌头原碱对非酒精性脂肪肝大鼠瘦素胰岛素抵抗的实验研究

目的:研究乌头原碱对大鼠非酒精性脂肪肝模型瘦素胰岛素抵抗的影响。方法:80只SD大鼠随机分为4组,每组20只。正常组喂普通饲料;模型组、治疗组和对照组喂高脂饲料。治疗组12周后给予乌头原碱2 mg/kg体质量灌胃治疗,对照组12周后给予二甲双胍150 mg/kg体质量灌胃治疗。16周处死大鼠。测定血清总胆固醇(total cholesterol,TC)、三酰甘油(triacylglycerol,TG),用放免法检测大鼠血清瘦素(leptin,LP)和胰岛素(in-sulin,Ins)水平,并观察肝组织脂肪变程度。结果:模型组血清TC、TG、LP、Ins水平升高,肝脏呈明显脂肪性变。治疗组较模型组及对照组血清TC、TG、LP、Ins水平均有显著性降低,脂肪性变程度明显降低(P<0.05)。结论:乌头原碱能通过调节TG、TC代谢,降低血清瘦素水平,改善胰岛素抵抗状态,降低肝脏脂肪性变,具有良好的防治脂肪肝作用。

Effect of aconine on leptin insulin resistance in rats with nonalcoholic hepatosteatosis

Objective: To study the effect of aconine on rat model of nonalcoholic hepatosteatosis.Methods Sixty male SD rats were divided into four groups randomly: thenormal group(n =20) fed with normal diet,the model group(n =20),the control group(n =20) and the treatment group(n =20) fed with high-fat diet for 16 weeks.Furthermore,the treatment group was given aconine(2 mg/kg ) simultaneously after 12 weeks of high-fat diet.The treatment group was given dimethylbiguanide(150 mg/kg) simultaneously after 12 weeks of high-fat diet.Sixteen weeks later,all rats were killed.Total cholesterol(TC),triacylglycerol(TG),leptin(LP),insulin(Ins) were detected,and to observe axungia degree of the liver.Results: In the model group,serum TC,TG,LP,Ins were markedly increased,the liver present fatty.Compared with the control group and the model group,in the treatment group,the value of serum TC,TG,LP,Ins were decreased,the degree of fat degeneration was remarkably.Conclusion: aconine can attenuate hepatic fat degeneration of rats with fatty liver induced by high-fat diet through decreasing TG,TC deposit in liver.