复方葛根素对糖尿病肾病Ⅳ型胶原的作用

目的 观察复方葛根素对糖尿病大鼠肾小球Ⅳ型胶原(IV-C)表达的影响.方法 利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠按照数字表法随机分为正常对照组(10只)、糖尿病组(10只)、葛根素治疗组(10只)、维生素C治疗组(10只)、维生素C和葛根素联合治疗组(10只).治疗12周,观察治疗期间及治疗后大鼠的一般状况、血糖、尿素氮、血肌酐,内生肌酐清除率、糖化血红蛋白、血清Ⅳ-C、24 h尿蛋白排泄率,免疫组化检测肾组织Ⅳ-C蛋白表达,原位杂交检测肾组织Ⅳ-Cα1基因表达.结果 (1)4个糖尿病大鼠模型组大鼠均出现肾脏功能损害;(2)葛根素和维生素C能改善基本状况,降低糖尿病大鼠(葛根素治疗组、维生素C治疗组、联合治疗组)的尿素氮(8.68±0.43)、(7.98±0.47)和(5.76±0.82)μmol/L]、血肌酐(74.68±8.20)、(75.52±7.98)和(58.66±6.65)mmol/L]、24 h尿白蛋白排泄率(18.40±0.37)、(17.24±0.30)和(9.97±1.27)mg/24 h×10-3];增加内生肌酐清除率(0.59±0.21)、(0.61±0.14)和(0.69±0.32)ml/min];Ⅳ-C、(111.56±14.61),吸光度显著下调(110.78±9.69)和(95.44±9.97)].结论 复方葛根素可能通过抑制Ⅳ-C的表达,具有确切的肾脏保护作用.

Effect of compound Puerarin on the collage Ⅳ in streptozotocin-induced diabetic nephropathy rats

Objective To observe the effect of compound Puerarin on collagen IV of streptozotocininduced diabetic rats.Methods Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin(STZ).Rats were allocated randomly to control group(10),diabetes model group(10),Vitamin C group(10),Puerarin group(10),vitamin C plus Puerarin group(10).The S tudy period lasted for 12 weeks.During and after the treatment,the general state,blood glucose levels,glycosylated hemoglobin,blood urea nitrogen,serum collagen IV,blood urea nitrogen,serum creatinine,urinary albumin excretion rate of the 24-hour,and clearance rate of creatinine collagen IV protein were determined by immunohistochemistoche analysis as well as type the gene expression of collagen IVα1 mRNA were determined by in situ hybridization analysis in the kidney tissue of different groups.Results (1)Diabetes mellitus and renal function lesion occurred in the four groups.(2)Vitamin C and Puerarin could improve the general conditions of diabetic Rats,decrease blood urea nitrogen(8.68±0.43),(7.98±0.47)and (5.76±0.82)umol/L,serum creatinine(74.68±8.20),(75.52±7.98)and(58.66±6.65)mmol/L],and urinary albumin excretion rate of the 24-hour(18.40±0.37),(17.24±0.30)and(9.97±1.27)mg/24 h×10-3];increase clearance rate of creatinine(0.59±0.21),(0.61±0.14)and(0.69±0.32)ml/min],the expression of collage IV absorbance(111.56±14.61),(110.78±9.69)and(95.44±9.97)]in the diabetic Rats were significantly inhibited at the same time.Conclusion The compound Puerarin might have some functions on preventing ren by inhibiting expression of type IV collagen.