Contribution of CD8+ T cells to innate immunity: IFN-gamma secretion induced by IL-12 and IL-18 |
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Authors: | Berg Rance E Cordes Christoph J Forman James |
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Affiliation: | Center for Immunology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, NA2.200, Dallas, TX 75390-9093, USA. |
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Abstract: | The role of CD8+ T cells in adaptive immunity is well documented and involves numerous effector mechanisms including direct cytolysis of targets and secretion of cytokines. The role of CD8+ T cells in innate immunity has not been previously appreciated. Using J774 macrophages infected in vitro with the intracellular bacterium, Listeria monocytogenes (LM), we show that CD8+ T cells isolated from na?ve C57BL/6 (B6) mice respond rapidly by secreting IFN-gamma. CD8+ T cells secreting IFN-gamma can also be found in na?ve B6 mice 16 h after infection with LM. This rapid IFN-gamma response is TCR-independent and mediated through the actions of IL-12 and IL-18. Cell surface staining and cell sorting experiments indicate that these novel CD8+ T cells express memory markers. In vitro CFSE-labeling experiments show that IFN-gamma-secreting CD8+ T cells proliferate rapidly after 2 days in culture and after 4 days constitute the majority of the CD8+ T cell population. Together, these data suggest an important role for IFN-gamma-secreting CD8+ T cells in the innate response to bacterial pathogens. |
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