| 浙南汉族人群18号染色体上三个STR位点的遗传多态性研究 |
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| 引用本文： | 张洪勤,吴淑珍,毕云天,包其郁. 浙南汉族人群18号染色体上三个STR位点的遗传多态性研究[J]. 中国优生与遗传杂志, 2005, 13(11): 13-15 |
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| 作者姓名： | 张洪勤 吴淑珍 毕云天 包其郁 |
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| 作者单位： | 1. 温州医学院生命科学院
2. 温州医学院临床学院,浙江,温州,325027 |
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| 基金项目： | 浙江省温州市科技局资助项目，浙江省教育厅资助项目 |
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| 摘 要： | 目的研究浙南汉族人群18号染色体上三个STR位点D18S865、D18S1364和D18S535的遗传多态性分布,获得相应的群体遗传学数据.方法应用聚合酶链式反应(PCR)、聚丙烯酰胺凝胶电泳(PAGE)及银染显带技术对浙南地区汉族184名无关个体的D18S865、D18S1364和D18S535位点进行分型.结果D18S865观察到6个等位基因及17种基因型,D18S1364观察到9个等位基因及35种基因型,D18S535观察到8个等位基因及29种基因型,多态性分布都符合Hardy-Weinberg平衡.各基因座的杂合度(H)分别为:0.723、0.848、0.783;多态性信息含量(PIC):0.72、0.81、0.79.结论三个STR基因座具有较高杂合度和多态性信息含量,在遗传学研究和法医学应用中有较高的价值.
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| 关 键 词： | 短串联重复序列 聚合酶链反应 遗传多态性 |
| 文章编号： | 1006-9534（2005）11-0013-03 |
| 收稿时间： | 2005-04-15 |
| 修稿时间： | 2005-04-15 |
Genetic polymorphism of 3 STR loci on chromosome 18 in Wenzhou Han population |
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| ZHANC Houg - qin, WU Shu - zhen, BI Yun - tian, et al. Genetic polymorphism of 3 STR loci on chromosome 18 in Wenzhou Han population[J]. Chinese Journal of Birth Health & Heredity, 2005, 13(11): 13-15 |
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| Authors: | ZHANC Houg - qin WU Shu - zhen BI Yun - tian et al |
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| Affiliation: | Molectdar Biology Research Centre of Wenzhou Medical College, Wenzhou 325027 |
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| Abstract: | Objective: To investigate genetic polymorphism of D18S865,D18S1364 and D18S535 in South Zhejiang Han population. Methods: Population studies on D18S865,D18S1364 and D18S535 were carried out in a sample of 184 unrelated South Zhejiang Han individuals using PCR followed by PAGE and silver stain detection. Results: 6,9,8 alleles and 17,35,29 genotypes of D18S865, D18S1364 ,D18S535 were respectively observed among the 184 unrelated individuals. The genotype frequency match the Hardy- Weinberg equilibrium, The heterozy-gotes(H) in D18S865,D18S1364 and D18S535 were 0.723,0.848,0.783, polymorphism information content(PIC) were 0.72,0.81,0.79 respectively. Conclusion: D18S865,D18S1364 and D18S535 had high heterozygotes and polymorphism information content, so the three loci can be used in genetic researches and in forensic medicine |
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| Keywords: | Short tandem repeats Polymerase chain reaction Genetic polymorphism |
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