大鼠肝移植肝动脉重建方法改进的实验研究

目的 构建重建肝动脉血供的大鼠肝移植模型.方法 在经典"二袖套"法的基础上,利用24G静脉留置针外鞘管制作动脉支架管吻合供体腹腔动脉及受体肝总动脉.结果 共进行肝移植26次.所有手术均单人操作完成.手术成功率(存活24 h以上)为92.3%(24/26).动脉吻合时间仅需(1.70±0.46)min.经解剖发现术后第1天及第30天所有动物肝动脉均通畅,搏动良好,各肝叶入口处动脉亦充盈良好.未发现胆道坏死,胰腺坏死或由于周围组织包绕形成的胆道内漏等.结论 利用支架法吻合供体腹腔动脉及受体肝总动脉的方法 是一种操作简便、耗时短、对正常生理干扰小、通畅率高的理想的大鼠肝移植肝动脉重建方法 .

Modified rearterialization in rat liver transplantation

Objective To establish a new method for hepatic arterial reconstruction in rat liver transplantation.Methods Based on the two cuffmethod,celiac artery of donor and the common hepatic artery of recipient were anastomosed with a splint which is made of intraventous catheter 24 G.All animals were killed to evaluate occlusion rates of the reconstructed arteries 1d and 30d after transplantation.Result A total of 26 cases of rat liver transplantation were performed by one person.The survival rate(survival time＞24 h)was 92.3%(24/26).The time expenditure to finish arterial anastomosis was only 1.70±0.46 min.The arteries of all animals were patent 1d and 30d after reperfusion.There was no necrosis of bile duct and pancreas,bile leakage and internal fistulae.Conelusion Anastomosing celiac artery of donor and the common hepatic artery of recipient by a splint is an ideal method of rearterialization in rat liver transplantation because of its simplicity,convenience and high rate of perfusion.