一氧化氮与大鼠肠缺血再灌注损伤关系的研究 |
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引用本文: | 胡博,詹江华,崔华雷,谷继卿. 一氧化氮与大鼠肠缺血再灌注损伤关系的研究[J]. 陕西医学杂志, 2007, 36(1): 29-31 |
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作者姓名: | 胡博 詹江华 崔华雷 谷继卿 |
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作者单位: | 天津儿童医院小儿外科,天津,300074 |
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摘 要: | 目的了解一氧化氮(NO)与大鼠肠缺血再灌注损伤之间存在的关系。方法制作肠缺血再灌注损伤的动物模型,设立对照组、缺血组、再灌注45min和90min组,检测不同时点血浆NO浓度、肠组织一氧化氮合酶(iNOS)免疫组化染色光密度及肠管病理损伤程度。结果伴随肠缺血再灌注进程,各组大鼠Chiu病理评分及iNOS免疫组化染色光密度值依次升高,且组间均有显著性差异。F值分别为287.13,62.484,P均小于0.01。而血浆NO含量则是在再灌注后才呈现渐增趋势,F=38.667,P<0.01。结论肠组织中iNOS表达在缺血期即被激活,由iNOS催化合成的NO在大鼠肠缺血再灌注过程中基本以致损作用为主。
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关 键 词: | 再灌注损伤/血液 一氧化氮/代谢 一氧化氮合酶/代谢 肠 动物 实验 大鼠 |
修稿时间: | 2006-03-06 |
A study on the relationship between NO and intestine ischemia-reperfusion injury in rats |
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Affiliation: | Tianjin 300074 |
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Abstract: | Objective: To observe the relationship between NO and intestine ischemia-reperfusion injury in rat. Methods: Establish intestine ischemia-reperfusion animal model. Wistar rats were randomly divided into four groups: contral group,ischemia group,reperfusion 45 minutes group and reperfusion 90 minutes group. Plasma NO content,expression of iNOS and intestine Chiu pathologic grade were measured at different time. Results:Both Chiu pathologic grade and expression of iNOS were increasing gradually and significantly during ischemia-reperfusion period(F=287.13,P<0.01; F=62.484,P<0.01). NO increased remarkably just in reperfusion phase(F=38.667,P<0.01). Conclusion: The expression of iNOS was activated in ischemia phase,and enhanced in reperfusion phase. NO, which was catalyzed by iNOS was intestine ischemia-reperfusion injury. |
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Keywords: | Reperfusion injury/blood Nitric oxide/metabolism Nitric-oxide synthase/metabolism Intestines Animals laboratory Rat |
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