恶性淋巴瘤患者外周血T细胞亚群的研究

 目的　检测恶性淋巴瘤患者外周血T细胞亚群，探讨其与恶性淋巴瘤发生、发展的关系。方法　采用XL型流式细胞仪检测106例恶性淋巴瘤患者及106例对照组外周血T细胞亚群，进行统计学分析。结果　恶性淋巴瘤患者外周血CD3及CD4／CD8数量水平均明显低于对照组（P＜0.05）。97例非霍奇金淋巴瘤（NHL）中，Ⅰ、Ⅱ期患者CD3降低，CD8升高（P＜0.05），而Ⅲ、Ⅳ期患者CD3、CD4、CD4／CD8均明显低于对照组（P＜0.05）。结论　恶性淋巴瘤患者细胞免疫功能降低，且随病情进展而加重。检测患者外周血T细胞亚群对评估患者的细胞免疫功能，了解其与肿瘤发生、发展的关系具有积极的临床意义。

Study of the counts of T-lymphocyte subgroup in patients with malignant lymphoma

Objective To detect T-lymphocyte subgroup in patients with malignant lymphoma, and investigate the relationship between the number of the cells and the oncogenesis and development of malignant lymphoma. Methods The counts of T-lymphocyte subgroup in peripheral blood of 106 patients with lymphoma were detected by flow-cytometry, and analyzed in comparison with that of 106 normal people as control. Results The counts of CD+3 cell and the ratio of CD+4/CD+8 in patients with malignant lymphoma were significantly lower than that of control group (P＜0.05). The number of CD+3 were significantly lower and CD+8 were higher than that of control group in patients with NHL at early ( Ⅰ, Ⅱ ) stage (P＜0.05). The counts of CD+3 CD+4 cell and the rate of CD+4/CD+8 in patients with NHL at advanced (Ⅲ ,Ⅳ) stage were significantly ldwer than that of control group (P＜0.05). Conclusion The function of immune is decreased in patients with malignant lymphoma, and these changes had correlation with clinic stage. It is of active significance by detecting T-lymphocyte subgroup in peripheral blood in patients with malignant lymphoma to evaluate the function of immune and find out the relationship between the number of the immune cells and the oneogenesis and development of malignant lymphoma.