| siRNA干扰沉默GS基因抑制胃癌细胞生长的研究 |
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| 引用本文： | 彭春伟,任峰,燕敏,俞焙秦,陈雪华,李建芳,刘炳亚,朱正纲.siRNA干扰沉默GS基因抑制胃癌细胞生长的研究[J].外科理论与实践,2010,15(2):148-152. |
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| 作者姓名： | 彭春伟 任峰 燕敏 俞焙秦 陈雪华 李建芳 刘炳亚 朱正纲 |
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| 作者单位： | 上海交通大学医学院附属瑞金医院外科,上海消化外科研究所,上海市胃肿瘤重点实验室,上海,200025 |
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| 基金项目： | 上海市重大科技专项(09DZ1950100); 上海市科委重点课题(07jc14041) |
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| 摘 要： | 目的：利用RNA干扰技术,研究靶向Glutamine Synthetase（GS）基因的小干扰RNA（siRNA）在体外对胃癌细胞生长的影响,探索胃癌基因治疗并了解GS在胃癌发生、发展中的作用。方法：合成GSsiRNA,并用脂质体法将GSsiRNA转至胃癌BGC-823细胞中;采用实时定量PCR和Western印迹法观察GSsiRNA转染前后胃癌细胞GS基因及相应蛋白表达的变化;用CCK8、流式细胞检测技术分别检测胃癌细胞增殖及凋亡的变化。结果：转染GSsiRNA后的胃癌细胞BGC-823与对照组相比,生长明显变缓（P〈0.05）,细胞凋亡率明显增高（P〈0.05）。结论：靶向GSsiRNA可明显下调靶基因GS的表达,在体外可抑制胃癌BGC-823细胞的生长并促进其凋亡。
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| 关 键 词： | 胃肿瘤 RNA 小干扰 基因 GS |
Experimental study on silencing the GS expression by siRNA to inhibit the growth of gastric cancer cells |
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| PENG Chun-wei,REN Feng,YAN Min,YU Pei-qin,CHEN Xue-hua,Li Jian-fang,LIU Bing-ya,ZHU Zheng-gang.Experimental study on silencing the GS expression by siRNA to inhibit the growth of gastric cancer cells[J].Journal of Surgery Concepts & Practice,2010,15(2):148-152. |
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| Authors: | PENG Chun-wei REN Feng YAN Min YU Pei-qin CHEN Xue-hua Li Jian-fang LIU Bing-ya ZHU Zheng-gang |
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| Institution: | PENG Chun-wei,REN Feng,YAN Min,YU Pei-qin,CHEN Xue-hua,LI Jian-fang,LIU Bing-ya,ZHU Zheng-gang.Department of Surger,Shanghai Institute of Digestive Surgeryy,Shanghai key Laboratory of Gastric Neoplasms,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China |
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| Abstract: | Objective To determine the inhibitory effect of synthetic GS short interfering RNA(siRNA) on the expression of GS gene in human gastric cancer line BGC-823 cells;and to elucidate its effect on the proliferation of BGC823 cells in vitro,in order to probe the feasibility of gene therapy in this domain.Methods siRNA targeting GS mRNA was transfected into BGC-823 cells,and GS expression was determined by real-time PCR and Western blot.Cell proliferation and apoptosis were examined by CCK8 and flow cytometry,res... |
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| Keywords: | Gastric neoplasms RNA small interfering Genes GS |
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