| 反相高效液相色谱法测定怀牛膝中羟基促蜕皮甾酮的含量 |
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| 引用本文： | 马英,车镇涛,毕开顺,王玺,黄伟.反相高效液相色谱法测定怀牛膝中羟基促蜕皮甾酮的含量[J].药学学报,2000,35(4):313-315. |
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| 作者姓名： | 马英 车镇涛 毕开顺 王玺 黄伟 |
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| 作者单位： | 1. 沈阳药科大学,沈阳,110015
2. 香港中文大学中医学院,香港 |
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| 摘 要： | 怀牛膝系苋科植物牛膝 (ＡｃｈｙｒａｎｔｈｅｓｂｉｄｅｎｔａｔａＢｌ.)的干燥根1] 。研究表明 ,怀牛膝含有皂苷、甾体类、糖类、氨基酸、生物碱类和香豆素类化合物 ,并含有钾盐及多种微量元素。甾体类化合物有蜕皮激素和植物甾醇 ,蜕皮激素包括羟基促蜕皮甾酮(ｅｃｄｙｓｔｅｒｏｎｅ ,ＥＤＳ)和牛膝甾酮。其中ＥＤＳ有促进蛋白质合成、抑制药物引起的血糖升高、改善肝功和降低血浆胆固醇的作用。目前 ,怀牛膝有效成分含量测定多以皂苷元齐墩果酸为指标 ,用薄层比色法、薄层扫描法、超临界流体色谱法等2～ 4 ] ,而蜕皮激素的含量测定报…
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| 关 键 词： | 怀牛膝 羟基促蜕皮甾酮 反相高效液相色谱法 |
| 收稿时间： | 1999-07-07 |
| 修稿时间： | : |
DETERMINATION OF ECDYSTERONE IN THE ROOT OF ACHYRANTHES BIDENTATA BL.BY RP-HPLC |
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| Ma Ying,Che Zhuntao,Bi Kaishun,Wang Xi,Huang Wei.DETERMINATION OF ECDYSTERONE IN THE ROOT OF ACHYRANTHES BIDENTATA BL.BY RP-HPLC[J].Acta Pharmaceutica Sinica,2000,35(4):313-315. |
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| Authors: | Ma Ying Che Zhuntao Bi Kaishun Wang Xi Huang Wei |
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| Abstract: | AIM: To set up an assay method for the determination of ecdysterone (EDS) in the root of Achyranthes bidentata Bl. by RP-HPLC. METHODS: The EDS was separated on a Hypersil-ODS column (200 mm×4.6 mm ID, 5 μm), using acetonitrile — water (1.0∶5.4) as the mobile phase and detected at 243 nm. The flow rate was 1.0 mL.min-1. Phenol was used as an internal standard. RESULTS: The calibration curve was in good linearity over the range of 28.04～280.40 μg (γ=0.9997). The average recovery was 95.2%. CONCLUSION: The method is simple, fast, sensitive and is suitable for quantitative analysis of EDS in the root of Achyranthes bidentata Bl. |
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| Keywords: | Achyranthes bidentata BL ecdysterone RP-HPLC |
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