高效液相色谱法测定四环素眼膏的含量

目的建立高效液相色谱法测定四环素眼膏中四环素的含量。方法采用BDS C18色谱柱（250mm×4．6mm，5μm），以0．05mol·L^-1草酸铵溶液-二甲基甲酰胺-磷酸氢二铵（75：20：5）为流动相，检测波长为280nm，流速为1．0ml·min^-1。结果四环素的峰面积与浓度呈良好的线性关系，线性范围为0．01—0．25g·L^-1，回归方程：Y=2．14×10^7X＋1．46×10^4，r=0．9999，n=7。平均回收率为99．5％，RSD=0．72％。分析方法精密度RSD=1．15％（n=5）。结论该方法简便、快速、准确。

Determination of ointment eye tetracycline by HPLC

Aim To establish a HPLC method for the determination of ointment eye tetracycline. Methods BDS C18 was used. The mobile phase consisted of Ammonium oxalate solution, dimethylformamide solution and diammonium hydrogen phosphate solution （75：20： 5, v/v）. The flow rate was 1.0 ml·min^-1, and the detection wavelength was set at 280 nm. Results The calibration carve was linear within the range of 0.01 and 0.25 g·L^-1 for the tetracycline,and the regression equation was Y=2.14×10^7X ＋ 1.46×10^4 ,r =0. 999 9 ,n = 7. The average recovery rate was 99.5%, and RSD was 0.72%. The method precision RSD was 1.15% （ n = 5 ）. Conclusion The method was rapid, simple and accurate.