丁酸钠对胰腺癌BxPC-3细胞凋亡机制的影响

目的:探讨丁酸钠对胰腺癌BxPC-3细胞凋亡的影响及其作用机制。方法:采用MTT法观察不同浓度丁酸钠对BxPC-3细胞增殖的抑制作用;应用流式细胞仪检测丁酸钠作用于BxPC-3细胞后的凋亡情况;TRAP-ELISA法检测细胞端粒酶活性的变化;RT-PCR技术分析人端粒酶逆转录酶(hTERT)、bcl-2 mRNA的表达水平。结果:丁酸钠作用BxPC-3细胞能明显抑制细胞增殖,其作用具有时间和剂量依赖性。丁酸钠处理72 h的BxPC-3细胞凋亡率明显提高(P<0.01),S期细胞比例显著下降(P<0.05),G0/G1期细胞比例显著升高(P<0.05)。经丁酸钠处理的实验组端粒酶活性为0.96±0.12,未经丁酸钠处理的对照组端粒酶活性为4.18±0.34,二者之间差异有显著性(P<0.05)。丁酸钠处理的实验组hTERT mRNA水平为0.168±0.045,与对照组0.685±0.141比较差异有统计学意义(P<0.01),bcl-2实验组水平为0.138±0.034,与对照组0.715±0.026比较有统计学差异。结论:丁酸钠具有强烈诱导胰腺癌BxPC-3细胞凋亡的作用,其发生机制与丁酸钠下调hTERT Bcl-2 mRNA表达水平直接相关。

Effects of Sodium Butyrate on the Apoptosis of Human Pancreatic Cancer Cell Line BxPC-3

Objective: To explore the effect of sodium butyrate on the cell apoptosis of human pancreatic cancer cell line BxPC-3 and its mechanism.Methods: The proliferating activity of BxPC-3 cells was observed by MTT assay.Flowcytometry was used to assess the apoptosis after sodium butyrate treatment.And TRAP-ELISA was applied to analyze the telomerase activity of human pancreatic cancer cell.In addition,the expressions of telomerase hTERT mRNA and Bcl-2 mRNA were confirmed by RT-PCR technology.Results: A time and dose dependent inhibition was remarkably confirmed in BxPC-3 cells.As compared with that in control group,apoptosis rate of BxPC-3 cells in sodium butyrate group was increased obviously at 72 h(P<0.01).The percentage of S phase decreased significantly,while the percentage of G0/G1 phase increased markedly(both P<0.05).The activity of telomerase in research group was significantly lower than that in control group(0.96±0.12 vs 4.18±0.34,P<0.05).Furthermore,the expression level of hTERT mRNA and bcl-2 mRNA in sodium butyrate group was significantly lower(0.168±0.045 vs 0.685±0.141,0.138±0.034 vs 0.715±0.026,respectively,both P<0.01).Conclusion: Sodium butyrate can greatly induce apoptosis ofpancreatic cancer BxPC-3 cell line,and the decline of expression level of hTERT Bcl-2 mRNA might contribute to the procedure of apoptosis.