异体脐血干细胞移植导致胃肠移植物抗宿主病的实验研究

目的观察心肌梗死(MI)大鼠接受静脉异体脐血干细胞(HUCBC)移植后发生腹胀的原因。方法大鼠于心肌梗死后5 d经尾静脉注入0.5 ml脐血[含单个核细胞(1～3)×107]或0.5 ml磷酸缓冲盐溶液(PBS)。不使用免疫抑制剂。观察8周,入选移植后发生腹胀的大鼠、未腹胀大鼠、对照(PBS)组大鼠(每组n=8),行超声心动图和血流动力学检查,同时对扩张肠道进行HE染色明确腹胀原因。结果梗死后移植大鼠心功能明显优于PBS大鼠,而腹胀与非腹胀大鼠相比,射血分数(EF)、短轴缩短率(FS)、左室压力最大变化率(±dp/dtmax)、左室舒张末压(LVEDP)没有显著差异,因此腹胀的发生与心功能恶化及移植效果没有关系。肠道大体解剖和病理分析显示移植大鼠(包括腹胀和未腹胀大鼠)均存在肠道扩张,以回肠周围最为明显,组织学表现符合肠道移植物抗宿主病。结论异体脐血单个核细胞移植可以导致胃肠道移植物抗宿主反应。

Human umbilical cord blood cells transplantation induced gastrointestinal graft versus host disease

Objective To study the cause for abdominal distention in rats with myocardial infarction （MI） after transplantation with hu- man umbilical cord blood cells （HUCBCs）. Methods Rats received HUCBCs （ 1 - 2 ×10^7 ） or an equal volume of PBS at 5 days after MI. The immunosuppressive agent had not been used. Eight weeks later, 8 transplanted rats suffered with abdominal distension, and PBS rats hadnt such symptom.. Cardiac function and distended small intestine in rats of transplanted group and control group were examined. Results HUCBCs could slightly improve ejection fraction （EF）, fractional shortening （FS）, and dp/dtmax in MI rats. The pathological examination of intestine in both distended and undistended rats demonstrated the presence of distention of intestine especially in ileus, the findings of histopathologieal examination coincided with those of gastrointestinal graft - versus - host disease （ GI - GVHD）. Conclusion HUCBCs can induce gastrointestinal graft versus host disease.