化学发光法检测CA125和CA153在卵巢肿瘤诊断中的临床意义

目的探讨化学发光法检测糖类抗原125(CA125)和糖类抗原153(CA153)在卵巢肿瘤诊断中的临床意义。方法选择2010年8月至2011年11月期间,妇科收治的卵巢肿瘤患者79例,其中恶性卵巢肿瘤患者39例,良性卵巢肿瘤患者40例,另选同期妇科进行非卵巢手术患者80例作为对照组。所有受检者均为空腹采集外周静脉血2.5～3.0 ml,采用罗氏Cobas e411全自动电化学发光免疫分析仪及配套试剂盒对样品CA125和CA153含量进行检测。对各组患者CA125和CA153的检测结果以及阳性结果进行比较分析。结果与对照组患者相比,恶性和良性卵巢肿瘤组患者CA125和CA153的水平均明显提高,差异具有统计学意义(P<0.05)。与健康对照组患者相比,恶性和良性卵巢肿瘤组患者CA125和CA153的阳性率明显提高,差异具有统计学意义(P<0.05)。结论采用化学发光法检测CA125和CA153对于卵巢癌的诊断均具有一定的特异性。在进一步的研究中,可以采用联合检测,以期待提高诊断的准确性。

Study on clinical significance of serum levels of CA 125 and CA 153 in diagnosis of ovarian tumors

Objective To explore the clinical significance of serum levels of CA 125 and CA 153 in diagnosis of ovarian tumors.Methods Seventy nine patients with ovarian tumors including 39 with malignant ovarian tumor and 40 with benign ovarian tumor in Department of Gynecology of this hospital during August 2010 to November 2011 were enrolled in this study,and 80 patients with non-ovarian gynecological operation at the same period were selected as control group.Fasting blood samples（2.5~3.0 ml） were collected in all these patients for detection of serum levels of CA 125 and CA 153 by using Roche Cobas e411 automatic chemiluminescence immunoassay analyzer and supporting kit.The results of serum levels of CA 125 and CA 153 in patients of both groups were compared and analyzed.Results In comparison with healthy controls,the serum levels of CA 125 and CA 153 in patients with malignant and benign ovarian neoplasms were significantly elevated,their difference was statistically significant（P0.05）.At the same time,the positive rates of CA 125 and CA 153 in patients with malignant and benign ovarian neoplasms were significantly higher than those of healthy controls,and their difference was statistically significant（P0.05）.Conclusion The detection of serum levels of CA 125 and CA 153 by using chemiluminescence immunoassay in diagnosis of ovarian cancer has certain specificity.In further study,joint detection of these two tumor markers may raise the accuracy of diagnosis.