球形幽门螺杆菌体外细胞粘附性研究球形Hp系列研究之一

通过采用延长培养时间和亚抑菌浓度抗生素的作用，使幽门螺杆菌（Ｈｐ）发生球形变异。电镜下观察球形Ｈｐ结构，并对球形Ｈｐ进行尿素酶活性测定和Ｈｅｐ－２细胞粘附试验，电镜下计算粘附率、粘附指数、侵袭率、侵袭指数。结果表明球形Ｈｐ具有完整的细胞壁，它不是Ｌ型细菌，尿素酶活性降低或消失，细胞粘附能力减弱，侵袭力与正常Ｈｐ无差异。以上两种方法诱变产生的球形Ｈｐ对细胞的粘附能力无差异。

Adherence of Coccoid Form of Helicobacter pylori to Epithelial Cells in vitro

The coccoid forms of H. pylori,which were transformed from bacillary by prolonged incubation or exposure in antibiotics with subinhibitory concentrations,were involved in this study.We found that the coccoid H.pylori didn't belong to L type bacteria,as its cell wall was observed to be integrated by transmission electron microscopy.We also testified that the urease activity of coccoid H. pylori was decreased or lost,and the ability of H.pylori to adhere to epithelial cells was abated obviously.The results suggested that the pathogenic power of coccoid H.pylori was weakened,although the invading capability was constant.