经皮瘤内注射32P-玻璃微球治疗肝癌剂量与生物效应的实验研究

Objection:To study the relationship between different doses and biological effect of 32P-glass microspheres(32P-GMS) by percutaneous intra-tumor injection at different times and provide proofs of theory for clinical therapy.Methods:36 Zealand rabbits and Vx-2 were used to establish the animal model of liver tumor.Six groups were randomly designed.The suspension of different radiative doses of 32P-GMS combined with lipiodol-ultrafluid(0.1 mL) was respectively injected by percutaneous intra-tumor.The tumor tissues were examined by light microscope.MRI examination of liver tumors were performed before and after the injection.Results:C and D groups were observed that the tumor volume was decreased and the rate of restrained tumor was gradually increased after injection of 32P-GMS.The living tumor tissues of E group completely disappeared after the injection for two weeks.MRI examination showed that the tumor signal of E group was equal as T2 as the signal of normal liver parenchyma.The living tumor tissues were not found in F group after the injection for three weeks.Conclusion:111 MBq was the best radiative dose of 32P-GMS for treatment of 1 cm liver cancer by percutaneous intra-tumor injection.MRI examination was very valuable to evaluate the result and follow up after the injection to treat liver cancer.

Experimental research of relationship between doses and biological effect of 32P-GMS by percutaneous intra-tumor injection to treat liver cancer

Objection: To study the relationship between different doses and biological effect of 32P-glass microspheres (32P-GMS) by percutaneous intra-tumor injection at different times and provide proofs of theory for clinical therapy. Methods: 36 Zealand rabbits and Vx-2 were used to establish the animal model of liver tumor. Six groups were randomly designed. The suspension of different radiative doses of 32P-GMS combined with lipiodol-ultrafluid (0.1 mL) was respectively injected by percutaneous intra-tumor. The tumor tissues were examined by light microscope. MRI examination of liver tumors were performed before and after the injection. Results: C and D groups were observed that the tumor volume was decreased and the rate of restrained tumor was gradually increased after injection of 32P-GMS. The living tumor tissues of E group completely disappeared after the injection for two weeks. MRI examination showed that the tumor signal of E group was equal as T2 as the signal of normal liver parenchyma. The living tumor tissues were not found in F group after the injection for three weeks.Conclusion: 111 MBq was the best radiative dose of 32P-GMS for treatment of 1 cm liver cancer by percutaneous intra-tumor injection. MRI examination was very valuable to evaluate the result and follow up after the injection to treat liver cancer.