补肾方药的不同组分对骨质疏松大鼠的影响

目的 :观察补肾方药的不同组分对骨质疏松大鼠多指标的影响。方法 :每周 2次肌肉注射地塞米松 0.1mg·100g^-1体重 ,加喂食低钙饲料 (钙含量 0.2% ) ,饮用蒸馏水 ,诱发大鼠骨质疏松。模型成功后 ,应用经不同浓度醇提处理的补肾方药的不同组分进行治疗 ,之后取血和骨组织 ,观察骨质疏松大鼠的血生化指标及骨形态计量学参数的变化。结果 :补肾方药的A ,B ,C ,D各组分对大鼠一般状况的改善程度 ,对血钙、磷、碱性磷酸酶 (ALP) ,甲状旁腺素 (PTH) ,降钙素 (CT) ,CT/PTH ,雌二醇 (E₂ ) ,睾酮 (T) ,T/E₂ 和骨组织形态计量学等多指标的影响 ,均不及总组分。结论 :补肾方药经处理后 ,可能破坏或损失了某些有效成分或是破坏了其整体集合作用 ,而使各组分的治疗效果均不如总组分为佳。

The Influence of the Different Components of Nourishing Kidney Herbs on Osteoporosis Rats

Objective: To observe the influent of the different components of nourishing kidney herbs on the main items of bone metabolism in osteoporosis rats induced with Dexamethasona(DXM). Method:Models of three month old SD female rats with osteoporosis here made by being fed with low calcium diet(containing calcium 0.2%) and distilled water,and injected with DXM 0.1 mg/100 g weight intramuscularly , twice a week. Then the osteoporosis rats were treated with different components of nourishing kidney herbs, and the change of calcium,phosphate,alkaline phosphatase(ALP),calcitonin(CT),PTH,CT/PTH,estrogen(E 2),testosterone(T) ,T/E₂ and bone section and bone quantitative morphology in these osteoporosis rats were observed. Result:The total components of nourishing kidney herbs could improve the general condition of osteoporosis rats, decrease PTH, increase CT, estrogen, testosterone, CT/PTH and T/E₂. The total components of nourishing kidney herbs could improve osteoporotic state, promote bone formation, and inhibite bone resorption. But no effect of the A,B,C,D components of nourishing kidney herbs on the main items of bone metabolism in osteoporosis rats induced with DXM was found. Conclusion :It is possible that the purification and separation of these herbs weaken or destroy the integrative effect of nourishing kidney herbs or destroy effective components of nourishing kidney herbs during the process of purification and separation .