胃癌中caspase-3表达及其临床病理意义

目的　研究caspase 3表达在胃癌发生发展中的作用。 方法　采用免疫组化S P法观察 113例胃癌旁上皮细胞、原发灶癌细胞和浸润淋巴细胞以及转移灶癌细胞中caspase 3表达 ,并分析原发灶癌细胞中caspase 3表达与胃癌临床病理特征的关系。结果　caspase 3在 5 0 4 % (5 7/113)的胃癌旁上皮细胞中表达 ,高于原发灶癌细胞中的阳性率 32 7% (37/113) (P <0 0 5 ) ;caspase 3在胃癌原发灶浸润淋巴细胞中的阳性率为 70 8% (80 /113) ,高于胃癌旁上皮细胞和原发灶癌细胞中阳性率(P <0 0 5 ) ;5 8 1% (2 5 /43)的转移灶癌细胞中caspase 3表达阳性 ,其阳性率高于对应原发灶癌细胞 (34 2 % ,13/38) (P <0 0 5 ) ;原发灶癌细胞中caspase 3表达与胃癌患者的年龄、性别以及胃癌肿瘤大小、浸润深度、转移、TNM分期、生长方式和分化程度无相关性 (P >0 0 5 )。结论　胃癌原发灶癌细胞中caspase 3表达下调和浸润淋巴细胞中caspase 3表达上调与胃癌发生有关 ,来自原发灶和转移微环境中的化学物质可能通过提高转移灶癌细胞中caspase 3表达 ,进而起到抑制转移灶的作用。

Caspase-3 expression and its clinicopathological significance in gastric cancer

Purpose To study the role of caspase-3 expression in carcinogenesis and progression of gastric cancer. Methods Epithelial cells of mucosa adjacent to carcinoma, cancer cells and infiltrating lymphocytes of primary foci and cancer cells of metastatic foci from 113 gastric cancers were studied using streptavidin-biotin-immunoperoxidase immunohisto chemistry with anti-caspase-3 antibody. Caspase-3 expression in cancer cells of primary foci was compared with the clinicopathological features of gastric cancer. Results There was high immunohistoreactivity to caspase-3 in epithelial cells of mucosa adjacent to carcinoma from 50.4%(57/113)of cases of gastric cancers. Despite no correlation with tumor size, invasive depth, metastases, differentiation, TNM staging, age and gender of patients (P>0.05), the positive immunoreactivity to caspase-3 in cancer cells of primary foci was found in 32.7%(37/113)of gastric cancer, less than that in adjacent epithelial cells (P<0.05). Infiltrating lymphocytes in primary foci showed strong immunoreactivity in 70.8%(80/113) of cases, more than adjacent epithelial cells or cancer cells of primary foci (P<0.05). Caspase-3 expression in metastatic cancer cells was more frequently expressed in 58.1%(25/43) of metastasis than in cancer cells of corresponding primary foci (34.2%, 13/38) (P<0.05). Conclusions Down-regulation of caspase-3 expression in cancer cells of primary foci and up-regulation of caspase-3 expression in their filtrating lymphocytes are associated with carcinogenesis of gastric cancer. Chemicals derived from primary foci and metastatic microenvironment might inhibit growth of metastatic cancer by increasing caspase-3 expression.