重组人乙型肝炎病毒S基因表达的体内外评价

目的 构建能表达乙型肝炎病毒（HBV）S基因的DNA疫苗，并在离体与活体条件下评价重组S基因的表达。方法 将克隆的S-X基因片段插入真核细胞表达载体。获得重组质粒，在离体转录系统与转染细胞系中表达重组S基因。并在小鼠中评价HBsAg激发抗-HBs的效果。结果 S基因的表达已通过Northern印迹杂交，Western印迹杂交和ELISA（抗原及抗体检测）予以证实。结论 S基因已成功地实现体外重组与表达。

Evaluation on expression of recombinant S gene of human hepatitis B virus in vitro and in vivo

Objective To construct a DNA vaccine capable of expressing S gene of hepatitis B virus(HBV) and evaluate the expression of recombinant S gene in vitro and in vivo. Methods A cloned S X gene fragment was inserted into a eukaryote expression vector to construct a recombinant plasmid. The S gene was transcribed in vitro and expressed in a transfected cell line, and the efficiency of HBsAg in eliciting anti HBs was evaluated in mice. Results The expression of S gene was confirmed by Northern blotting, Western blotting, and ELISA(for both antigen and antibody detection). Conclusions The recombination and expression of S gene is achieved successfully in vitro.