甲氨基阿维菌素的致突变性、致畸性及亚慢性毒性的研究

目的与方法:本文报道了甲氨基阿维菌素小鼠骨髓嗜多染红细胞微核试验,小鼠精子畸形试验,Ames 试验,对大鼠的亚慢性毒性试验及致畸试验的研究结果。结果与结论:其剂量在6. 3～25. 2mg/ kg 的微核试验结果表明,各剂量组与阴性对照组的微核率比较,结果无显著意义( P > 0105) 。10～500μg/ 皿剂量组Ames 试验及15. 8～63mg/ kg. bw 剂量组小鼠精子畸形试验结果均为阴性。致畸研究结果表明,该药对大鼠无明显母体毒作用和胚胎毒作用。亚慢性毒性试验中,高剂量组雌性大鼠(9. 26mg/ kg. bw) 尿素氮、雄性大鼠(12. 6mg/ kg. bw) 尿素氮及白蛋白水平有一定程度的改变,雄、雌性大鼠肾脏脏器系数有显著的改变( P < 0105) 。雄性大鼠(12. 6mg/ kg. bw) 体重明显降低( P < 0105) ,比雌性大鼠影响略大。亚慢性毒性最大耐受剂量雌性大鼠为1. 16mg/ kg. bw ,雄性大鼠为1. 57mg/ kg. bw。

STUDIES ON THE MUTAGENICITY, TERATOGENICITY AND SUBCHRONICTOXICITY OF METHYLAMINO-ABAMECTIN

Purpose and Methods : Mutagenicity , teratogenicity and subchronic toxicity in mice or rat s induced by Methylamino2Abamectin (MA) were studied. Results and Conclusion : In comparison with negative cont rol , each dosage did not show significant differences in the rates of marrow cell micronuclei and spermatic deformity of mice ( P > 0. 05) ; but revealed significant difference compared to positive cont rol ( P < 0. 05) . MA (102500μg/ plate) didn’t induce positive mutations of st rains TA97 ,TA98 ,TA100 and TA102 with or without S9mix n Ames test . It suggested that MA has no obvious mutagenicity and embryotoxic or teratogenic effects in Wistar rats. At the highest dosage , BUN and Albumin at 12. 6mg/ kg. bw in females showed significant diffence ( P <0. 05) , so did BUN at 9. 26mg/ kg. bw in males ( P < 0. 05) . Body weight s were decreased in males and organ coefficient of kidney was increased in males ad females. The maximum no2observed2effect level of MA in subchromic toxicity was 1. 16mg/ kg. bw and 1. 57mg/ kg. bw in males and females , respectively.