| 逆转录－巢式聚合酶链反应检测庚型肝炎病毒RNA方法的建立及初步应用 |
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| 引用本文： | 李伯安,程云,罗清华,郭仁峰,马洪滨,张建宗,曹阳,付体权,薛静,李靖. 逆转录－巢式聚合酶链反应检测庚型肝炎病毒RNA方法的建立及初步应用[J]. 中华实验和临床病毒学杂志, 1996, 0(3) |
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| 作者姓名： | 李伯安 程云 罗清华 郭仁峰 马洪滨 张建宗 曹阳 付体权 薛静 李靖 |
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| 作者单位： | 北京解放军３０２医院免疫研究室 |
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| 摘 要： | 庚型肝炎病毒（ＨＧＶ）基因组为单链、正股ＲＮＡ，全长９３９２ｂｐ。根据５’－非编码区基因序列设计合成两对引物。随机选取酶联抗－ＨＧＶ阳性病人血清３份，阴性病人血清６份，应用逆转录－巢式聚合酶链反应进行检测。结果２份抗－ＨＧＶ阳性血清可见较强的特异扩增带，１份抗－ＨＧＶ阳性血清可见较弱的特异扩增带，其余６份抗－ＨＧＶ阴性血清均无特异性扩增带。特异扩增片断大小与设计相符，并经序列分析及Ｓｏｕｔｈｅｒｎ杂交给予证实。
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| 关 键 词： | 庚型肝炎病毒，聚合酶链反应，RNA，病毒。 |
DetectionofhepatitisGvirus(HGV)byreversetranscriptasenestedpolytnerasecbainreactionanditspre-limlnaryapplication |
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| LiBoan,ChengYun,LuoQinghua,et al.. DetectionofhepatitisGvirus(HGV)byreversetranscriptasenestedpolytnerasecbainreactionanditspre-limlnaryapplication[J]. Chinese journal of experimental and clinical virology, 1996, 0(3) |
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| Authors: | LiBoan ChengYun LuoQinghua et al. |
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| Affiliation: | LiBoan,ChengYun,LuoQinghua,etal..DepartmentofImmunologyofImmunology302Hos-pitalofPLA,Beijing.100039 |
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| Abstract: | The.HepatitisGvirus(HGV)isalinearpositive-strandedRNAandmoleculeof9393bpencodingapolyproteinprecursorof2873aminoacidsThe5'untranslatedregion(5,UTR)asthetargetforPCR,twoptirsofprimersweredesigned.Threeserafromanti-HGVpositiveliverdiseasepatientsandsixsamplesfromanti-HGVnegativeliverdiseasepatientswereselectedrandomlyfordetectionofHGVRNA.3PCRamplifiedproductsfrom3anti-HGVposi-tiveserawereidentifiedandnoneofthe6anti-HGVnegativeserumrevealedPCRpositiveamplification.Theamplifiedproductswerefragmentsabout190bpinlengthandidenticaltodesignedfragments.DNAsequencingandSouthern-blotcon-firmedthatthenested-PCRprimerswereofHGV-specificitv.ThismethodhaspotentialvalueinrapiddiasnosisforHGVinfection. |
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| Keywords: | HepatitisGvirusPolymerasechainreactionRNA viral1LinnenJ WagesJJ ZHang-KeckZY etal.MolecularcloninganddiseaseassociationofhepatitisGvirus:atreansfu-sion-transmissibledisease.Science.1996 271(5248):505.2OhbaK MizokamiM LauJY etal.Evolutionaryrelation-shipof |
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