大鼠牙移动性根吸收组织内组织蛋白酶K及白细胞介素6 mRNA的表达

目的 检测正畸牙齿移动引起根吸收组织中组织蛋白酶K(CK)及白细胞介素6(IL-6)的分子表达水平及其分布,探讨根吸收过程的分子机制。方法 选用Wistar大鼠建立根吸收的动物模型,应用原位杂交技术观察CK和IL-6在根吸收组织中的定位表达,应用t检验对比二者在根吸收组织与正常牙周组织中表达的差异。结果 CK mRNA表达于破牙和破骨细胞内,而IL-6 mRNA主要表达于成纤维细胞、成骨细胞、骨细胞及成牙骨质细胞内。二者在牙根吸收组织中的表达均明显高于正常组织(P<0.01)。结论 CK表达于根吸收组织中的破牙细胞,作为一种最重要的蛋白质分解酶参与有机基质的降解;IL-6作为多功能细胞因子,在根吸收活动中起着重要的调控作用。

Expression of cathepsin K and IL-6 mRNA in root-resorbing tissue during tooth movement in rats

Objective To investigate the expression and the localization of Cathepsin K and IL-6 mRNA in root-resorbing tissue and to elucidate the molecular changes and mechanism of root resorption induced by tooth movement. Methods Rats were subject to experimental tooth movement to induce root resorption. In situ hybridization was performed to identify the cells in root-resorbing tissue that produced Cathepsin K or IL-6 the difference of CK mRNA or IL-6 mRNA expression between root resorption group and control group was calculated by t-test. Results Cathepsin K mRNA was highly and selectively expressed in multinuclear odontoclast and IL-6 mRNA expressed in fibroblast, osteoblast, osteocyte and cementoblast. The expression of Cathepsin K mRNA and IL-6 mRNA in root-resorbing tissue increased evidently compared with the normal periodontium. Conclusions Odonto clast in the root-resorbing tissue expresses Cathepsin K mRNA that participates in proteolysis during root resorption. IL-6 plays a very important role in the root resorption as a multifunctional cytokine.