绞股蓝总皂甙对单个豚鼠心室肌细胞的钙、钠、钾电流及动作电位的影响 |
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引用本文: | 赵颖,胡大一.绞股蓝总皂甙对单个豚鼠心室肌细胞的钙、钠、钾电流及动作电位的影响[J].中国药理学通报,1998,14(1):60-62. |
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作者姓名: | 赵颖 胡大一 |
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作者单位: | 首都医科大学附属北京红十字朝阳医院心脏中心 |
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摘 要: | 目的研究绞股蓝总皂甙(GP)对成年豚鼠单个心室肌细胞的动作电位(AP)、L-型钙通道电流(ICa)、快钠通道电流(INa)以及内向整流钾通道电流(IK1)的影响。方法膜片钳全细胞记录。结果50mgL-1GP能使动作电位时程(APD90)由给药前的(708±24)ms缩短到给药后的(396±16)ms(P<0.01),抑制率58.1%,动作电位幅值(APA)由给药前的(121±7.2)mV降到给药后的(86±8.6)mV(P<0.01),抑制率24.1%,静息膜电位无明显影响;5~50mgL-1的GP能浓度依赖性阻滞ICa和INa,但对IK1无明显影响。结论GP对缺血心肌的保护作用在于减少“钙超载”和抑制异常兴奋性的产生。
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关 键 词: | 绞股蓝总皂甙 膜片钳全细胞记录 心室肌细胞 动作电位 L-型钙电流 钠电流 内向整流钾电流 |
Effect of GP on action potentials,L calcium channel currents,sodium channel currents and potassium channel currents in isolated single ventricular myocyte of guinea pigs |
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Abstract: | AIM To investigate the effects of GP on action potentials, L calcium channel currents, sodium channel currents and potassium channel currents in isolated single ventricular myocyte of guinea pig. METHODS Whole cell recording by patch clamp technique. RESULTS GP 50 mg·L-1 shortened action potential duration (APD90) 581% and shortened action potential amplitude (APA) 241%, but had no significant effects on rest membrane potential (RMP). GP 5 ̄50 mg·L-1 inhibitted calcium currents and sodium currents. The inhibition was concentration dependent. GP did not effect inward rectified potassium currents. CONCLUSIONS GP might prevent cardial myocyte from ischemic damage by inhibitting the “calcium overload” and generation of abnormal excitement. |
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Keywords: | GP whole cell recording ventricular myocyte action potential L calcium channel currents sodium currents inward rectified potassium currents |
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