粉防己碱逆转肿瘤多药抗药性细胞的凋亡抗性作用

目的探讨粉防己碱逆转ＭＤＲ细胞凋亡抗性的作用及其机制。方法ＭＤＲ细胞株ＭＣＦ－７／Ａｄｒ与其相应的敏感株ＭＣＦ－７进行对比研究。比较粉防己碱对阿霉素（Ｄｏｘ）诱导ＭＤＲ细胞及其相应的敏感株的凋亡作用。并比较粉防己碱对ＭＤＲ细胞及其相应的敏感株的细胞ｂｃｌ－２蛋白的表达的影响。细胞凋亡及ｂｃｌ－２蛋白表达的测定以流式细胞仪法。结果加入Ｄｏｘ共同培养２４ｈ可诱导７４．６％ＭＣＦ－７细胞凋亡，而只引起１４．３％的ＭＣＦ－７／Ａｄｒ细胞凋亡。只加入粉防己碱共同培养２４ｈ，未见明显增加ＭＤＲ细胞及其相应敏感细胞的凋亡百分比。Ｄｏｘ＋粉防己碱能显著地使ＭＤＲ细胞的凋亡增至４７．０％，与单独用Ｄｏｘ组相比较，差异有显著性（Ｐ＜０．０１），而敏感细胞株为７７．８％，与单独用Ｄｏｘ组相比较，差异无显著性（Ｐ＞０．０５）。ＭＤＲ细胞株与其相应的敏感株ｂｃｌ－２蛋白表达水平均较低且差异无显著性。加入粉防己碱对ＭＤＲ细胞株与其相应的敏感株ｂｃｌ－２蛋白表达水平均未见明显影响。结论粉防己碱能逆转ＭＤＲ细胞对Ｄｏｘ的凋亡抗性。其逆转机制可能与ｂｃｌ－２蛋白表达无关。粉防己碱逆转ＭＤＲ细胞ＭＣＲ－７／ＡＤＲ对Ｄｏｘ的凋亡抗性的机制有待进?

Reversal action of apoptotic resistance in multidrug resistant cell line by tetrandrine

AIM To study the reversal action of apoptotic resistance in multidrug resistant (MDR) cell lines by tetrandrine(TTD) and mechanism involved in it. METHODS They were compared that the rates of apoptosis in human breast cancer cell line MCF 7/ADR which were reistant to adriamycin(Dox) and their parental sensitive cell line MCF 7 induced by Dox in the presence or absence of TTD. And the change of expression of bcl 2 protein in the presence of TTD was determined. The rates of apoptosis and expression of bcl 2 protein were measured by flow cytometry. RESULTS The apoptotic rates induced by Dox in MCF 7/ADR cells and MCF 7 cells were 74 6% and 14 3%, respectively. This suggested that MDR cells MCF 7/ADR were resistant to apoptosis induced Dox. The apoptotic rate was increased in MCF 7/ADR cells in the presence of TTD, but no in MCF 7 cells. This demonstrated that TTD could reverse the apoptotic resistance in MDR cells. TTD could not change the expression of bcl 2 protein in MCF 7/ADR cells and MCF 7 cells. CONCLUSION TTD could reverse the apoptotic resistance to Dox in MCF 7/ADR cells and its mechanism was not involved in the expression of bcl 2 protein.