前列腺素E_1、川芎嗪合用对大鼠心肌缺血再灌注损伤的保护作用

目的观察前列腺素Ｅ１（３１２５μｇ·ｋｇ－１）与川芎嗪（２５ｍｇ·ｋｇ－１）合用对大鼠心肌缺血再灌注损伤的影响。方法麻醉大鼠冠脉结扎３０ｍｉｎ后，再灌６０ｍｉｎ诱发心律失常，硫代巴比妥酸法和分光光度法分别测定ＭＤＡ、ＳＯＤ及ＧＳＨ Ｐｘ，原子吸收光度法测定心肌细胞内Ｃａ２＋含量。结果两药小剂量合用的效果优于各药较大剂量单用。联合用药不仅更显著提高缺血再灌心肌ＳＯＤ、ＧＳＨ Ｐｘ活力（Ｐ＜００１），尚可显著降低ＭＤＡ、Ｃａ２＋及血清ＣＫ ＭＢ含量（Ｐ＜００１），防止缺血再灌室性心律失常的发生（Ｐ＜００１）。结论前列腺素Ｅ１与川芎嗪合用对心肌缺血再灌注损伤的保护作用有显著的协同作用。其作用与提高自由基清除酶活性、抑制脂质过氧化反应和防止心肌细胞内“钙超负荷”有关

. Protective effect of prostaglandin E 1 combined with ligustrazine on myocardial ischemia reperfusion injury in rats

AIM To study the effects of prostaglandin E 1 (PGE 1) and ligustrazine(LT), alone or in combination, on reperfusion arrhythmias in anesthetized rats. METHODS The arrhythmias were produced by coronary artery ligation for 30 min followed by reperfusion 60 min. Superoxide dismutase(SOD) and glutathione peroxidase (GSH Px) activities, malondialdehyde(MDA) and Ca 2+ contents in myocardium, serum creatine Kinase MB(CK MB) were measured by thiobarbituric acid fluorescence assay, colorimetric determination and atomic absorption photometry. RESULTS PGE 1 (31 15 μg·kg -1 ) in combination with LT (25 mg·kg -1 ) were more effective on prevention of the reperfusion arrhythmias than each drug alone at more dose. The combination of both drugs not only decreased the contents of MDA, Ca 2+ and CK MB, but also increased the activity of SOD and GSH Px in reperfusion myocardium. CONCLUSION PGE 1 in combination with LT in half of individual dose has protective effects on myocardial ischemia reperfusion injury and there was some synergic effect when they were used in combination. The protective effect may be from that they can increase the activity of clearence enzyme of free radical, inhabit lipid peroxidation, and the calcium influx.