爱大霉素和庆大霉素对肾皮质线粒体~(45)Ca~(2+)摄取的影响

目的研究爱大霉素和庆大霉素对大鼠肾皮质线粒体钙摄取的影响。方法应用４５Ｃａ２＋示踪技术。结果两药在１ｍｍｏｌ·Ｌ－１时显著促进线粒体钙摄取（分别由每３０ｍｉｎ（１１３．５±７．５）μｍｏｌ·ｇ－１Ｐｒｏ上升到（１４８．０±２４．０）μｍｏｌ·ｇ－１Ｐｒｏ，（１１１．９±１３．０）μｍｏｌ·ｇ－１Ｐｒｏ上升到（１４０．１±１６．６）μｍｏｌ·ｇ－１Ｐｒｏ，Ｐ＜０．０５）；在１０μｍｏｌ·Ｌ－１时显著抑制线粒体钙摄取（分别由每３０ｍｉｎ（１１３．５±７．５）μｍｏｌ·ｇ－１Ｐｒｏ下降到（９２．３±５．７）μｍｏｌ·ｇ－１Ｐｒｏ，（１１１．９±１３．０）μｍｏｌ·ｇ－１Ｐｒｏ下降到（１４０．０±５．３）μｍｏｌ·ｇ－１Ｐｒｏ，Ｐ＜０．０５或Ｐ＜０．０１）。结论两药对线粒体钙摄取的这种双相变化可能与它们肾毒性有关

The effects of etimicin and gentamicin on renal corticalmitochondria ~(45)Ca~(2+) uptake

AIM To study the effects of etimicin and gentamicin on renal cortical mitochondria ~(45)Ca~(2+) uptake. METHODS Using ~(45)Ca~(2+) incorporation technique. RESULTS The two drugs at the concentration of 1 mmol·L-1 significantly promoted the ~(45)Ca~(2+) uptake 〔the former ascended from per 30 min(1135±75) to (1480±240) μmol·g-1 Pro, the latter from per 30 min(1119±130) to (1401±166) μmol·g-1 Pro, P<005〕, and at the concentration of 10 mmol·L-1, dramatically inhibited the mitochondria ~(45)Ca~(2+) uptake 〔the former descended from per 30 min(1135±75) to (923±57) μmol·g-1 Pro, the latter from per 30 min(1119±129) to (695±53) μmol·g-1 Pro, P<005 or P<001〕. CONCLUSION The two phase effects of the two drugs on mitochondria ~(45)Ca~(2+) uptake may correlate with their nephrotoxicity.