一种新二萜类化合物的体外抗肿瘤研究 |
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引用本文: | 崔燎,张晓.一种新二萜类化合物的体外抗肿瘤研究[J].中国药理学通报,1998,14(1):50-52. |
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作者姓名: | 崔燎 张晓 |
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作者单位: | 广东医学院药理教研室 |
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摘 要: | 目的研究一种新二萜类化合物凤厥内酯A(F-A)的抗肿瘤活性。方法采用体外培养的人癌细胞株——早幼粒细胞白血病HL-60和红白细胞白血病K562作对象,观察F-A对其抑瘤活性、诱导分化、细胞周期的影响。结果F-A对HL-60和K562细胞24h的半数抑制浓度IC50分别为7.6mgg-1和9.1mgg-1,在4mgL-1时即明显抑制HL-60细胞的对数生长;对3H-TdR参入作用在培养48h后有一定抑制作用;透视电镜显示肿瘤细胞有损伤表现;细胞周期分析显示G2+M期细胞增多;NBT还原反应试验阴性。结论F-A明显抑制HL-60和K562细胞生长,抗肿瘤活性强,对细胞周期的影响提示M期阻滞,研究初步提示F-A不具细胞诱导分化作用。
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关 键 词: | 二萜类 人白血病细胞株 抗肿瘤 细胞周期 诱导分化 |
Antitumor effects of a new diterpene in vitro |
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Abstract: | AIM To determine the antitumor effects of a new diterpene (petrisolide A, F A) against human promyeloxytic leukemia HL 60 and erythroleukemia K562 cell lines in vitro. METHODS Antitumor activity was measured using IC50, cell growth curve and 3H TdR uptake. Simultaneously, the cell cycle was analysed by flow cytometric(FCM) technique and the induced differentiation was tested by nitronblue tetrazolium(NBT) red uction. RESULTS IC50 of F A was 76 mg·L-1 to HL 60 and 91 mg·L-1 to K562 respectively when incubation for 24 h. It obviously inhibited the growth curve of HL 60 at 4 mg·L-1, the assays of 3H TdR incorporation into DNA showed a slight inhibition and the morphology was showed a cell damage under transmission electron microscopy (TEM) in the tumor cells of treated group. FCM analysis revealed that F A could arrest HL 60 cells to G2+M phase and the S and G1 phase were not altered. In the tests of NBT reduction in the presence of F A at 4 mg·L-1 for 4 days, HL 60 cells did not show NBT reductive ability. CONCLUSION F A remarkbly inhibited HL 60 and K562 cell lines proliferation and showed a high antitumor activity. It was suggested that F A might inhibite mitoses of the cells and have not ability to induce differentiation. |
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Keywords: | diterpene leukamia cell lines antitumor cell cycle induced differentiation |
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