地黄寡糖对3T3-L1脂肪细胞增殖及胰岛素抵抗的作用

目的：探讨地黄寡糖对脂肪细胞增殖和胰岛素抵抗的影响。方法：培养3T3-L1前脂肪细胞,用四甲基偶氮唑盐（MTT）方法检测3T3-L1前脂肪细胞及脂肪细胞的增殖情况,同时采用地塞米松诱导3T3-L1脂肪细胞建立胰岛素抵抗模型,检测地黄寡糖对细胞培养基中葡萄糖浓度的影响。结果：在DMEM高糖培养基中,地黄寡糖可促进3T3-L1前脂肪细胞增殖,抑制3T3-L1脂肪细胞增殖,作用呈明显量效关系；使3T3-L1前脂肪细胞及3T3-L1脂肪细胞葡萄糖消耗量增加,呈明显量效关系；地黄寡糖能明显增加胰岛素抵抗3T3-L1脂肪细胞培养基中的葡萄糖消耗量,增强对胰岛素的敏感性。结论：地黄寡糖可以促进前脂肪细胞的增殖,抑制脂肪细胞的增殖,地黄寡糖对地塞米松诱导的3T3-L1脂肪细胞胰岛素抵抗具有明显的改善作用。

Effects of Rehmannia glutinosa oligosaccharides on proliferation of 3T3-L1 adipocytes and insulin resistance

Objective: To investigate the influence of Rehmannia glutinosa oligosaccharides(ROS) on the proliferation of 3T3-L1 adipocytes and insulin resistance.Method: 3T3-L1 preadipocytes were cultured,the proliferation of 3T3-L1 preadipocytes was detected by MTT method.Insulin resistant 3T3-L1 adipocytes cell model was induced by dexamethasone and the change of glucose concentration in cell culture was determined after ROS treatment.Result: In the high glucose DMEM culture media,MTT method showed that the absorbance at 570nm of 3T3-L1 preadipocytes was increased and that of 3T3-L1 adipocytes was decreased.ROS significantly increased glucose consumption in 3T3-L1 preadipocytes and adipocytes culture in a concentration-dependent manner. ROS improved the sensitivity of 3T3-L1 adipocytes to insulin.Conclusion: ROS can promote the proliferation of 3T3-L1 preadipocytes,inhibite the proliferation of 3T3-L1 adipocytes,and also,significantly improve insulin resistance induced by dexamethasone.