心肌梗死患者对氧磷酶-1 Q/R192基因多态性及活性检测的临床意义

目的探讨心肌梗死（AMI）患者对氧磷酶-1（PON1）Q/R192基因多态性及其活性检测的临床意义。方法分别采用紫外线分光光度法和聚合酶链式反应-限制性片段长度多态性（PCR-RELP）法检测65例AMI患者和70名健康体检者PON1活性及PON1Q/R192基因多态性。结果 AMI组血清PON1活性（78.56±16.69）U/mL]明显低于健康对照组（118.65±30.25）U/mL]（P〈0.01）。AMI组与健康对照组3种基因型及2种等位基因频率分布差异无统计学意义（P〉0.05）;AMI组与健康对照组间不同PON1 Q/R192基因型间血清PON1活性相比差异有统计学意义（P〈0.01）;AMI组内及健康对照组内PON1 Q/R192不同基因型间血清PON1活性相比差异无统计学意义（P〉0.05）。结论血清PON1活性降低是AMI的危险因素之一;PON1Q/R192基因多态性与AMI的发生无相关性。

Clinical significance of detecting Q/R192 polymorphism of the paraoxonase-1 gene and its activity in patients with acute myocardial infarction

Objective To study the clinical significance of Q/R192 polymorphism of the paraoxonase-1（PON1） gene and its activity in patients with acute myocardial infarction（AMI）.Methods PON1 activity was detected by ultraviolet spectrophotometry,and the Q/R192 polymorphism of the PON 1 gene was determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RELP） in 65 patients with AMI and 70 healthy controls.Results Serum PON1 activity （78.56 ± 16.69） U/mL] in AMI patients was significantly lower than that in healthy controls （118.65 ± 30.25） U/mL]（P0.01）.The frequency distribution of 3 genotypes and 2 alleles had no significant difference（P0.05）.The serum PON1 activity of different PON1 Q/R192 genotypes was significantly different between AMI patients and healthy controls（P0.01）.The serum PON1 activity of different PON1 Q/R192 genotypes had no statistically significant difference in the groups of AMI patients and healthy controls（P 0.05）.Conclusions The decrease of serum PON1 activity is one of the risk factors for AMI.PON1Q/R192 gene polymorphism has no correlation with AMI.