前列环素合酶基因多态性与新疆维吾尔族心肌梗死的相关性

目的 探讨前列环素合酶基因C1117A多态性与新疆维吾尔族心肌梗死的相关性.方法 采用聚合酶链反应.限制性片段长度多态性方法,对310例心肌梗死患者和306名健康受试者前列环素合酶基因第8外显子C1117A位点进行分析,同时进行血清6-酮前列腺素F1α水平测定.结果 前列环素合酶基因第8外显子C1117A在病例组和健康对照组中基因型频率分别为:CC型0.70和0.62,CA型0.26和0.32,AA型0.03和0.06,两组CC基因型频率差异具有统计学意义(P＜0.05),且病例组C等位基因频率高于对照组(P＜0.01),而CA和AA基因型差异无统计学意义(P＞0.05).血清6-酮前列腺素F1α水平病例组较对照组明显降低,差异具有统计学意义(P＜0.05);CC基因型携带者血清6-酮前列腺素F1α水平较CA和AA基因型者降低,差异具有统计学意义(P＜0.05).结论 前列环素合酶基因第8外显子C1117A基因多态性和新疆维吾尔族心肌梗死的发生具有相关性,可能和基因变异导致的血清前列环素水平降低有关.

Association of polymorphism of the prostaclin synthase gene with myocardial infarction in Uigur population of Xinjiang

Objective To investigate the association between the polymorphism of the prostacyclin synthase gene and Uigur patients with myocardial infarction in Xinjiang.Methods Three hundred and ten patients with myocardial infarction (MI) and 306 healthy control subjects were detected by polymerase chain reaction and restriction fragment length polymorphism.The serum 6-keto-PGF1α was detected with radioirmnunoassay kit in all subjects.Results The genotype distributions of the control group and MI group were in the Hardy-Weinberg equilibrium(X2=0.442,1.867,P＞0.05).The frequencies of CC,CA and AA were 0.70,0.26 and 0.03 in the MI group and 0.62,0.32 and 0.06 in the controis.There was significant difference in frequencies of CC genotype and C allele but no difference in frequencies of CA and AA genotypes between the controls and the MI cases.There was significant difference in serum 6-keto-PGF1α,level between the MI group and control group (P＜0.05),as well as among the three genotypes (P＜0.05).In the cases with CC genotype the serum 6-keto-PGF1α level was lower than that of others (P＜0.05).Conclusion The CC genotype and C allele of the prostacyclin synthase gene might be a risk factor of MI in Uigur population in Xinjiang,which may lead to the decreased serum 6-keto-PGF1α,level.