免疫介导再生障碍性贫血小鼠血清诱导骨髓造血细胞凋亡及？…

目的：探讨再生障碍性贫血（再障）小鼠骨髓造血细胞损伤的机制。方法：以重组的人ＧＭ－ＣＳＦ和ＩＬ－３作为造血细胞存活因子，实验组以再障小鼠血清加入正常小鼠骨髓细胞培养体系，同时设立正常小鼠血清培养组和无小鼠血清培养组两个平行对照组。结果：实验从超微结构、流式细胞仪及ＤＮＡ裂解分析三方面证实再障小鼠血清诱导骨髓细胞发生凋亡。在一定浓度范围内，随再障小鼠血清浓度的增高，其诱导ＤＮＡ裂解百分率逐渐增高。再

Induction of hematopoietic cell apoptosis by the serum from immune-mediated aplastic anemia mice]

OBJECTIVE: To explore the mechanism of the impairment of hematopoietic stem/progenitor cells in aplastic anemia(AA) mice. METHODS: The hematopoietic cells from normal mice bone marrow were incubated with serum from AA mice in the presence of recombinant GM-CSF and IL-3. Parallel controls, i.e. hematopoietic cells from normal mice with or without serum of normal mice, were conducted. At different time, samples were collected and detected by flow cytometry (FACS), transmission electron microscopy (TEM) and gel electrophoresis. RESULTS: In the 16 post hour-culture of the experimental group mice: (i) The percentages of apoptotic cells were increased. (ii) Hematopoietic cells showed nuclear condensation, margination and blebbing under TEM. (iii) Agarose gel electrophoresis of DNA from the 13,000x g supernatant showed a ladder pattern. The percentage of DNA fragmentation measured by diphenylamine reaction was also increased and (iv) ZnSO4, cycloheximide and actinomycin D could partially suppress the induction of DNA fragmentation and delay the ocurrence of apoptosis. CONCLUSION: Apoptosis occurs in hematopoietic cells when incubated with serum from immune-mediated AA mice.