| PTA1单克隆抗体诱导人血小板活化聚集和胞浆Ca^2＋水平的升高 |
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| 引用本文： | 孙忱,金伯泉.PTA1单克隆抗体诱导人血小板活化聚集和胞浆Ca^2＋水平的升高[J].中华血液学杂志,1998,19(3):133-137. |
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| 作者姓名： | 孙忱 金伯泉 |
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| 作者单位： | 第四军医大学免疫学教研室，中国医学科学院中国协和医科大学血液学研究所 |
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| 摘 要： | 目的：探讨血小板和Ｔ细胞活化抗原１（ＰＴＡ１）诱导人血小板活化聚集和对血小板胞浆Ｃａ２＋水平影响的机制。方法：血小板聚集与ＡＴＰ释放试验及血小板胞浆Ｃａ２＋水平测定。结果：ＰＴＡ１单克隆抗体（ＭｃＡｂ）体外可诱导人血小板活化聚集，ＥＧＴＡ与ＰＧＩ２可以完全抑制ＰＴＡ１ＭｃＡｂ诱导的血小板活化聚集，ＰＴＡ１ＭｃＡｂＦ（ａｂ′）２对ＣＤ９或ＣＤ４１诱导的血小板活化聚集无明显影响。ＰＴＡ１ＭｃＡｂ促进血小板胞浆Ｃａ２＋水平升高。结论：ＰＴＡ１ＭｃＡｂ的刺激作用与血小板膜表面Ｆｃ受体和ＣＤ４１／ＣＤ６１（ⅡｂⅢａ）复合物有关，促进血小板胞浆Ｃａ２＋水平升高为胞外Ｃａ２＋内流与胞浆内Ｃａ２＋储存释放共同作用所致。
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| 关 键 词： | 血小板 血小板和T细胞活化抗原 聚集 单克隆抗体 钙 |
PTA1 monoclonal antibody induces human platelet aggregation and intra-cytoplasmic Ca2+ elevation] |
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| C Sun,B Jin,X Liu.PTA1 monoclonal antibody induces human platelet aggregation and intra-cytoplasmic Ca2+ elevation][J].Chinese Journal of Hematology,1998,19(3):133-137. |
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| Authors: | C Sun B Jin X Liu |
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| Institution: | Department of Immunology, Fourth Military Medical University, Xian 710032. |
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| Abstract: | OBJECTIVE: To explore the mechanism of PTA1 monoclonal antibody (McAb) induced human platelet aggregation and its effect on intra-cytoplasmic Ca2+ level. METHODS: Platelet aggregation, ATP releasing assay and Pollock's test were used. RESULTS: PTA1 McAb induced human platelet aggregation in vitro, which could be completely inhibited by EGTA and PGI2. F(ab')2 of PTA1 McAb had no effect on CD9 or CD41 induced platelet activation and aggregation. PTA1 McAb enhanced platelet intra-cytoplasmic Ca2+ elevation. CONCLUSION: PTA1 McAb inducing platelet aggregation is related to platelet Fc receptor and CD41/CD61 (II b/III a) complex, and the induced platelet intra-cytoplasmic Ca2+ elevation is resulted from Ca2+ influx and releasing of intracytoplasmic Ca2+ storage. |
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| Keywords: | Platelet PTA1 Aggregation Monoclonal antibody Calcium |
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