| 维甲类化合物Ro13-7410对HL-60细胞凋亡及分化诱导作用 |
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| 引用本文： | 刘小珊,娄陵生,蒋纪恺.维甲类化合物Ro13-7410对HL-60细胞凋亡及分化诱导作用[J].中华血液学杂志,1998,19(5):241-243. |
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| 作者姓名： | 刘小珊 娄陵生 蒋纪恺 |
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| 作者单位： | 重庆医科大学临床医学院血液科，重庆医科大学临床生化教研室 |
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| 摘 要： | 目的：观察第三代维甲类化合物（Ｅ）４［２５，６，７，８ｔｅｔｒａｈｙｄｒｏ５，５，８，８ｔｅｔｒａｍｅｔｈｙｌ２ｎａｐｈｔｈａｌｅｎｙｌ１ｐｒｏｐｅｎｙｌ］ｂｅｎｚｏｉｃａｃｉｄ（Ｒｏ１３７４１０）对人白血病细胞系ＨＬ６０的影响。方法：应用剂量反应曲线，台盼蓝排斥法，光镜、电镜，凋亡细胞检测试剂盒以及硝基四氮唑蓝还原能力等测定观察Ｒｏ１３７４１０对ＨＬ６０细胞的作用。结果：Ｒｏ１３７４１０能明显抑制ＨＬ６０细胞增殖，并同时诱导ＨＬ６０细胞的凋亡和分化。结论：Ｒｏ１３７４１０作用于ＨＬ６０细胞后可使ＨＬ６０细胞发生凋亡和分化，其诱导的分化作用比同等浓度的全反式维甲酸弱
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| 关 键 词： | 细胞凋亡 细胞分化 人白血病细胞株 维甲类化合物 |
Induction of apoptosis and differentiation in human leukemia cell line HL-60 by synthetic retinoid Ro13-7410] |
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| X Liu,L Lou,J Jiang.Induction of apoptosis and differentiation in human leukemia cell line HL-60 by synthetic retinoid Ro13-7410][J].Chinese Journal of Hematology,1998,19(5):241-243. |
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| Authors: | X Liu L Lou J Jiang |
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| Institution: | Department of Hematology, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016. |
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| Abstract: | OBJECTIVE: To investigate the effect of the synthetic retinoid Ro13-7410 on HL-60 cells. METHODS: Trypan blue exclusion was used to determine viability. Apoptosis was assessed by cell morphology and PCD-assay-kit. Differentiation was assessed by morphology and NBT reduction. RESULTS: Incubation of the HL-60 cells with 10(-6)-10(-8) mol/L Ro14-7410 resulted in the suppression of cell growth, the induction of cell apoptosis and differentiation. CONCLUSION: Ro13-7410 suppressed HL-60 cell growth mainly via induction of apoptosis. |
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| Keywords: | Apoptosis Differentiation Human leukemic cell line Retinoids |
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